Figure 3.

GC-bias plots for the human genome. Left: the GC composition distribution of the human genome (HG19, GRCh37). Center and right: GC-bias plots for several data sets from human NA12878. Unbiased coverage would be represented by a horizontal line at relative coverage = 1. Center: HiSeq v3 with sample-preparation reagents from Kapa Biosystems (Table 2, data set 14), Ion Torrent PGM (data set 15), and Complete Genomics data (data set 16). Right: HiSeq v3 with sample-preparation reagents from Kapa Biosystems (data set 14, as in center panel) and HiSeq v3 with the standard Fisher et al. [31] reagents (data set 13). Note that Illumina relative coverage exceeded the y-axis above 93% GC content. Relative coverage is only plotted for GC percentages for which there are at least 1,000 100-base windows in the genome.