SVP regulates STIP and genes involved in cytokinin signaling. (a) Quantification by qRT-PCR of the STIP mRNA levels in svp-41 (dashed line) compared to Col-0 (solid line). svp-41 and Col-0 seedlings were grown for 10 and 14 days, respectively, under SDs and then shifted to LDs for 3 or 5 days; (b) expression levels of STIP in Col-0, ft-10 tsf-1, and ft-10 tsf-1 svp-1 genetic backgrounds. Seedlings were grown under SDs for 14 days and then shifted to LDs for 3 or 5 days. Bars in (a) and (b) show standard deviation of the mean of three biological repeats; (c) list of genes differentially expressed in svp-41 compare to Col-0 related to the CK signaling pathway; (d) comparison between genes affected by BA treatment (Brenner et al., 2005) and genes de-regulated in svp-41. For this analysis the genes represented in the affymetrix tiling Array 1.0R (used in the present study), but not represented in the ATH1 microarray used by Brenner et al. (2005), were excluded. A total of 167 genes were common between the two conditions (P = 6.6 × 10-13, based on the binomial test).
Gregis et al. Genome Biology 2013 14:R56 doi:10.1186/gb-2013-14-6-r56