SVP binds and regulates itself. (a) Binding profiles for SVP on SVP genomic locus in seedlings (upper panel) and inflorescence (lower panel) tissues. TAIR annotation corresponds to TAIR8. Grey boxes represent the region validated by ChIP-PCR in panels (b) and (c); (b) and (c) ChIP-PCR validations for two specific regions named I and II. ChIP assays were done using GFP antibodies and SVP::SVP-GFP svp-41 plants and compared to wild-type control plants. ChIP-PCR validation in vegetative (b) and reproductive tissue (c); (d) qRT-PCR expression analysis using primers for the SVP 3'UTR region. RNA was extracted from wild-type Col-0, svp-41, and SVP::SVP-GFP svp-41 seedlings and from wild-type Col-0, svp-41 agl24-2 ap1-12 triple mutant, and SVP::SVP-GFP svp-41 inflorescences; (e) qRT-PCR expression analyses using primers for the SVP 3'UTR region and coding region. RNA was extracted from wild-type Col-0, svp-41, and 35S::SVP seedlings. In all graphs error bars represent the standard deviation of normalized data (SD).
Gregis et al. Genome Biology 2013 14:R56 doi:10.1186/gb-2013-14-6-r56