Confirmation of individual histone methylation marks in primed roots. Position and verification of differential sites for H3K4me2 (green), H3K4me3 (red/yellow), and H3K27me3 (blue). Differences identified by CHIPDIFF are indicated with red bars above the ChIP-Seq profiles on the left (displayed in IGB). Profiles of non-primed roots (CR) are in shown in dark colors, those of primed roots (PR) in light colors. Black arrows indicate genome positions of the fragments amplified by qPCR. Average relative amounts of DNA amplified by qPCR for the indicated sites are shown in the bar graphs on the right. Each value was normalized against input and reference. References were constitutively di- or tri-methylated regions in At2g24560 (for H3K4) or At5g56920 (for H3K27). Bars are means ± SE of three independently treated replicate plant batches each consisting of approximately 300 plants (same material as pooled for ChIP-sequencing). Significant differences between primed and non-primed plants are indicated with * for P <0.05 and ** for P <0.01.
Sani et al. Genome Biology 2013 14:R59 doi:10.1186/gb-2013-14-6-r59