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Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana tomentosiformis

Nicolas Sierro, James ND Battey, Sonia Ouadi, Lucien Bovet, Simon Goepfert, Nicolas Bakaher, Manuel C Peitsch and Nikolai V Ivanov*

Author Affiliations

Philip Morris International R&D, Philip Morris Products SA, Quai Jeanrenaud 5, 2000 Neuchatel, Switzerland

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Genome Biology 2013, 14:R60  doi:10.1186/gb-2013-14-6-r60

Published: 17 June 2013

Additional files

Additional file 1:

Statistics of the Nicotiana sylvestris sequencing libraries.

Format: DOCX Size: 14KB Download file

Open Data

Additional file 2:

Statistics of the Nicotiana tomentosiformis sequencing libraries.

Format: DOCX Size: 14KB Download file

Open Data

Additional file 3:

Repetitive elements in the Nicotiana sylvestris and Nicotiana tomentosiformis genomes.

Format: DOCX Size: 21KB Download file

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Additional file 4:

Comparisons between the SSR mapping to the draft genomes and the PCR amplification test results.

Format: DOCX Size: 14KB Download file

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Additional file 5:

Genetic map of Nicotiana acuminata.

Format: DOCX Size: 23KB Download file

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Additional file 6:

Comparisons between the SSR mapping to the draft genomes and existing genetic maps.

Format: DOCX Size: 14KB Download file

Open Data

Additional file 7:

Genetic map of Nicotiana tomentosiformis.

Format: DOCX Size: 40KB Download file

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Additional file 8:

Comparisons between the COSII mapping to the draft genomes and existing genetic maps.

Format: DOCX Size: 14KB Download file

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Additional file 9:

Statistics of the Nicotiana sylvestris and Nicotiana tomentosiformis RNA-seq libraries.

Format: DOCX Size: 19KB Download file

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Additional file 10:

Transcriptome sequence number and length distribution.

Format: DOCX Size: 19KB Download file

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Additional file 11:

Genomic GO term (Biological Process) enrichment in Nicotiana sylvestris and Nicotiana tomentosiformis genes.

Format: DOCX Size: 17KB Download file

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Additional file 12:

Non-exhaustive list of gene copies potentially involved in cadmium/zinc (Cd/Zn) accumulation in Nicotiana leaves.

Format: DOCX Size: 27KB Download file

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Additional file 13:

Phylogenetic tree of CAX proteins from the N. sylvestris, N. tomentosiformis and Arabidopsis genomes. The N. sylvestris and N. tomentosiformis proteins are numbered according to the rows of 12. Bootstrap percentages are shown at each node.

Format: DOCX Size: 139KB Download file

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Additional file 14:

Key genes involved in the synthesis of nicotine and nornicotine alkaloids in Nicotiana leaves.

Format: DOCX Size: 31KB Download file

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Additional file 15:

Phylogenetic tree of PMT proteins from the N. sylvestris, N. tomentosiformis and N. tabacum genomes. The N. sylvestris and N. tomentosiformis proteins are numbered according to the rows of 14. Bootstrap percentages are shown at each node.

Format: DOCX Size: 116KB Download file

Open Data

Additional file 16:

Phylogenetic tree of CYP82E cytochrome P450 proteins and orthologs from the N. sylvestris and N. tomentosiformis genomes. The N. sylvestris and N. tomentosiformis proteins are numbered according to the rows of 14. Bootstrap percentages are shown at each node.

Format: DOCX Size: 187KB Download file

Open Data