Results of mapping-by-sequencing with outcross populations. Pools of 40 to 400 individuals (colored blocks) were sequenced with increasing coverage ranging from 5x to 200x. For each combination of pool size and coverage we simulated 500 independent populations and performed a mapping-by-sequencing analysis on each of them. Average mapping interval size and its standard deviation as well as the imputed number of candidate mutations within mapping region are shown on right and left y-axis, respectively. The initial number of mutations per genome was 1,400.
James et al. Genome Biology 2013 14:R61 doi:10.1186/gb-2013-14-6-r61