Results of mapping-by-sequencing with backcross populations. (A) Pools of 3 to 70 BC1F2 individuals (colored blocks) were sequenced with increasing coverage ranging from 5x to 200x. For each combination of pool size and coverage we simulated 500 independent populations and performed a mapping-by-sequencing analysis on each of them. Average number of candidate mutations with one standard deviation is shown on the y-axis. The initial number of mutations per genome was 1,400. (B) Zoom in on the framed region in panel A. Pools with three recombinants are not shown.
James et al. Genome Biology 2013 14:R61 doi:10.1186/gb-2013-14-6-r61