Table 1

Suggestions for the design of mapping-by-sequencing experiments

Outcross populations

Backcross populations

Direct sequencing

Deep candidate resequencing (dCARE)

Generation

F₂

BC₁F₂

BC_1-3F₂^a

n/a

Mutants (n)

Approximately 150

Approximately 50

1

As many as possible

Optimal coverage

>25

Approximately 50

>25

n/a

Sequencing type

Paired-end

Paired-end

Paired-end

Single-end

^aDepending on mutation rate.

James et al. Genome Biology 2013 14:R61   doi:10.1186/gb-2013-14-6-r61