Table 1

Suggestions for the design of mapping-by-sequencing experiments

Outcross populations

Backcross populations

Direct sequencing

Deep candidate resequencing (dCARE)


Generation

F2

BC1F2

BC1-3F2a

n/a

Mutants (n)

Approximately 150

Approximately 50

1

As many as possible

Optimal coverage

>25

Approximately 50

>25

n/a

Sequencing type

Paired-end

Paired-end

Paired-end

Single-end


aDepending on mutation rate.

James et al. Genome Biology 2013 14:R61   doi:10.1186/gb-2013-14-6-r61

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