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Analyzing the soybean transcriptome during autoregulation of mycorrhization identifies the transcription factors GmNF-YA1a/b as positive regulators of arbuscular mycorrhization

Sara Schaarschmidt12*, Peter M Gresshoff3 and Bettina Hause1

Author Affiliations

1 Leibniz Institute of Plant Biochemistry (IPB), Weinberg 3, 06120 Halle (Saale), Germany

2 Humboldt-Universität zu Berlin, Faculty of Agriculture and Horticulture, Division Urban Plant Ecophysiology, Lentzeallee 55-57, 14195 Berlin, Germany

3 ARC Centre of Excellence for Integrative Legume Research (CILR), The University of Queensland, St. Lucia, Queensland 4072, Australia

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Genome Biology 2013, 14:R62  doi:10.1186/gb-2013-14-6-r62

Published: 18 June 2013

Additional files

Additional file 1:

Supplemental Figures S1 to S10 and Supplemental Table S4: Figure S1. Procedure and plant photographs of split-root experiments. Figure S2. Mycorrhization phenotype of wild-type, nts382, and nts1007 in the split-root experiments II and III. Figure S3. Mycorrhization phenotype of wild-type, nts382, and nts1007 in dependence on phosphate and nitrate fertilization. Figure S4. Putative NARK-response genes identified by Affymetrix GeneChip analysis. Figure S5. Additional putative NARK-response genes in soybean plants 19 days after inoculation. Figure S6. Putative NARK-response genes in 7-week-old soybean plants. Figure S7. Affymetrix gene expression data of putative annexins 19 days after inoculation. Figure S8. Sequence information of the putative CCAAT-binding TF genes targeted by GmaAffx.40657.1.S1_at. Figure S9. Affymetrix gene expression data of other putative NF-Y genes in soybean plants 19 days after inoculation. Figure S10. Transcripts accumulation of the putative NF-YA genes Glyma10g10240 and Glyma02g35190 in root tissue upon R. irregularis-inoculation. Table S4. Sequences of primers used for RT-qPCR analysis and for creating the RNAi construct.

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Additional file 2:

Supplemental Tables S1 to S3: Table S1. Soybean genes found in the Affymetrix data to be locally regulated by R. irregularis 19 days after inoculation. Table S2. Soybean candidate genes selected by Affymetrix data for further analyses. Table S3. Affymetrix data of putative CCAAT binding TF genes of soybean.

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