Figure 3.

Effect of R. irregularis on local and systemic gene expression in wild-type and nts1007. (a) Number of genes regulated locally by R. irregularis in wild-type (wt) and nts1007 roots. (b, c) Number of genes regulated systemically by R. irregularis in shoots (b) and non-inoculated root-parts (c) of wild-type and nts1007 plants. Affymetrix gene expression analysis was done for plants of the split-root experiment I harvested 19 days after initial inoculation (see Figure 1). Left circles and right cycles indicate AM-regulated genes in wild-type and nts1007, respectively, whereas overlaying areas indicate genes that fulfill the criteria in both genotypes. Criteria for all comparisons were >2-fold changes with P ≤0.1 for all unpaired and paired t-tests performed with dChip (n = 3). ↑: upregulated genes ↓: downregulated genes.