Putative NARK-responsive genes in soybean plants 19 days after inoculation. Transcript accumulation of all genes is shown for shoots (left) and root-parts (right) of non-inoculated ('control') and partially R. irregularis-inoculated plants ('treatment'). Gene expression in mycorrhizal plants was separately analyzed for the inoculated root-part ('myc') and for the non-inoculated, autoregulated root-part ('non-myc'). (a) Glyma18g17440, putative ornithine acetyl transferase. (b) Glyma02g11150, Stress-induced receptor-like kinase 1 (GmSIK1). (c) Glyma17g09270, putative DEAD box RNA helicase. Relative transcript levels were determined by RT-qPCR in wild-type and nark mutant (nts382 and nts1007) plants harvested 19 days after partial inoculation with R. irregularis (for details see Figure 1). Data are mean values + SD with n = 9-18 and n = 8-15 for wild-type and nark mutants, respectively, coming from at least two independent experiments. Different letters indicate significant differences (P ≤0.05, multiple Student's t-tests with Bonferroni correction).
Schaarschmidt et al. Genome Biology 2013 14:R62 doi:10.1186/gb-2013-14-6-r62