Transcript accumulation pattern of two homologous annexins in wild-type and nark mutant plants. (a-c) Relative transcript levels of Glyma15g38010, predicted annexin named GmAnn1a (a, c), and Glyma13g26960, predicted annexin named GmAnn1b (b), in split-root plants of wild-type and nark mutants (nts382 and nts1007). Plants were harvested 19 days (a, b) and 40 days (c) after partial inoculation with R. irregularis (for details see Figure 1 and legend of Figure 4). Data are means + SD with n ≥10 and n ≥8 for wild-type and nark mutants, respectively, coming from at least two independent experiments. Different letters indicate significant differences (P ≤0.05, multiple Student's t-tests with Bonferroni correction). (d) Relative transcript levels of GmAnn1a in different tissues of non-inoculated 7-week-old wild-type and nark mutant (nts382 and nts1007) plants. Mean values + SD of plants of one experiment, wild-type: n = 5-6, nark mutants: n = 3. All transcript levels were determined by RT-qPCR and set in relation to GmSUBI-1 (a-d).
Schaarschmidt et al. Genome Biology 2013 14:R62 doi:10.1186/gb-2013-14-6-r62