Table 2

Transcript analysis of NF-YA and phosphate transporter genes in A. rhizogenes-transformed roots

EV

RNAi

Ratio

Gene

Mean

SD

Mean

SD

(RNAi/EV)


Glyma03g36140 (GmNF-YA1a)

0.21

±

0.097

0.084

±

0.048

0.40***

Glyma19g38800 (GmNF-YA1b)

0.19

±

0.099

0.082

±

0.055

0.44***

Glyma10g10240 (putative NF-YA)

0.021

±

0.014

0.017

±

0.015

0.80

Glyma02g35190 (putative NF-YA)

0.036

±

0.018

0.023

±

0.015

0.65**

Glyma13g08720 (phosphate transporter)

0.026

±

0.018

0.0098

±

0.0075

0.38***

Glyma14g28780 (phosphate transporter)

0.15

±

0.10

0.060

±

0.051

0.40***

Glyma14g36650 (phosphate transporter)

0.0026

±

0.0023

0.0010

±

0.0006

0.37***

RiBTub1

0.0036

±

0.0028

0.0014

±

0.0011

0.40***


Transcript levels of different NF-YA genes and AM-induced phosphate transporters were exemplified analyzed in chimeric plants with a Bragg wild-type background. Transcripts relative to GmSUBI-1 were determined by RT-qPCR using gene-specific primers (for primer information see Table S4 in Additional file 1). Specificity of primer pairs was assessed by sequencing of RT-qPCR products. Data are mean values + SD with n = 30 and n = 35 for EV and RNAi, respectively, coming from two independent experiments. Data of empty vector control and RNAi plants were pairwise compared by the Student's t-test. *P ≤0.05, **P ≤0.01, ***P ≤0.001. Significant changes are highlighted in bold.

EV: empty vector control; RNAi: roots expressing the RNAi construct.

Schaarschmidt et al. Genome Biology 2013 14:R62   doi:10.1186/gb-2013-14-6-r62

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