Additional file 10.

Figure S1. Assessment of cell viability during Phytophthora capsici infection. Transgenic Nicotiana benthamiana plants constitutively expressing ER-eGFP, a green fluorescent protein (GFP) localized to the endoplasmic reticulum (ER) were used to assess whether host cells were alive during the course of infection. (A) Photographs of N. benthamiana leaves infected with zoospore suspensions of P. capsici at 0, 8, 24, 48, and 72 hpi. (B) Confocal microscopy images of N. benthamiana leaves infected with a transgenic P. capsici strain expressing the fluorescent protein TdTomato. Within the first 24 hours, the host ER was largely intact despite the presence of P. capsici, and haustoria were often seen to invaginate living cells. After 24 hours, the ER network was disrupted as shown by the unstructured distribution of GFP, suggesting dead or dying cells. Bar = 20 μm.

Format: PNG Size: 5.9MB Download file

Jupe et al. Genome Biology 2013 14:R63   doi:10.1186/gb-2013-14-6-r63