Figure 6.

Occupancy of H2A.Z-nucleosomes is reduced at higher ambient temperatures in developing grain but not in vegetative seedlings. (a-c)ChIP analysis of HTA9:3XFLAG (H2A.Z) at 22°C and 27°C in vegetative seedlings at +1 nucleosomes of genes whose expression was up-regulated (a), remained constant (b) or down-regulated (c) upon an increase in temperature. (d-f)ChIP analysis of HTA9:3XFLAG (H2A.Z) at 22/17°C and 22/27°C in developing grain at +1 nucleosomes of genes whose expression was up-regulated (d) in both seedlings and grain, or remained constant (e) upon an increase in temperature. (f)ChIP analysis of HTA9:3XFLAG (H2A.Z) at 22/17°C and 27/22°C for genes with roles in grain development. Mock reactions (grey) were performed on identical tissue from wild-type plants. ***P < 0.001.

Boden et al. Genome Biology 2013 14:R65   doi:10.1186/gb-2013-14-6-r65
Download authors' original image