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This article is part of a special issue on plant genomics.

Open Access Highly Accessed Research

Mapping gene activity of Arabidopsis root hairs

Ping Lan1, Wenfeng Li1, Wen-Dar Lin1, Simonetta Santi2 and Wolfgang Schmidt134*

Author Affiliations

1 Institute of Plant and Microbial Biology, Academia Sinica, 11529 Taipei, Taiwan

2 Dipartimento di Scienze Agrarie e Ambientali, Universitá degli Studi di Udine, Via delle Scienze 206, I-33100 Udine, Italy

3 Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan

4 Genome and Systems Biology Degree Program, College of Life Science, National Taiwan University, Taipei, Taiwan

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Genome Biology 2013, 14:R67  doi:10.1186/gb-2013-14-6-r67

Published: 25 June 2013

Additional files

Additional file 1:

Figures S1-S8. Figure S1. Separation and size distribution of EXP7 protoplasts. Figure S2. Validation of RNA-sequencing (RNA-seq) results by quantitative reverse transcription (qRT)-PCR. Figure S3. Coexpression relationships of genes preferentially expressed in root hairs. Figure S4. Root-hair phenotypes of mutants harboring defects in the expression of genes from the coexpression network. Figure S5. Validation of alternative-splicing features by quantitative reverse transcription (qRT)-PCR. Figure S6. Scheme for the identification and quantification of proteins. Figure S7. Distributions of molecular weight and isoelectric point of proteins. Figure S8. The interactome of root hairs.

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Additional file 2:

Dataset S1. Read numbers and RPKM values from the transcriptome analysis.

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Additional file 3:

Dataset S2. Differentially expressed genes.

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Additional file 4:

Tables S1-S7

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Additional file 5:

Dataset S3. Splice junctions in EXP7 and non-GFP cells. GFP, green fluorescent protein.

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Additional file 6:

Dataset S4. Overview of alternative-splicing events in EXP7 and non-GFP cells. GFP, green fluorescent protein.

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Additional file 7:

Data set S5. Differential alternative-splicing events. Table S1. List of the root-related microarray experiments used for gene clustering. Table S2. Genes used for the search for expression signatures. Table S3. Post-translational modifications in the root protoplast proteome. Table S4. Root hair-specific proteins. Table S5. Differentially expressed proteins in EXP7 cells. Table S6. Confirmed interactions between root-hair proteins Table S7. Primers used for the validation of alternatively splicing features and differentially expressed genes.

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