Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Open Access Highly Accessed Research

Mapping gene activity of Arabidopsis root hairs

Ping Lan1, Wenfeng Li1, Wen-Dar Lin1, Simonetta Santi2 and Wolfgang Schmidt134*

Author Affiliations

1 Institute of Plant and Microbial Biology, Academia Sinica, 11529 Taipei, Taiwan

2 Dipartimento di Scienze Agrarie e Ambientali, Universitá degli Studi di Udine, Via delle Scienze 206, I-33100 Udine, Italy

3 Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan

4 Genome and Systems Biology Degree Program, College of Life Science, National Taiwan University, Taipei, Taiwan

For all author emails, please log on.

Genome Biology 2013, 14:R67  doi:10.1186/gb-2013-14-6-r67

Published: 25 June 2013

Additional files

Additional file 1:

Figures S1-S8. Figure S1. Separation and size distribution of EXP7 protoplasts. Figure S2. Validation of RNA-sequencing (RNA-seq) results by quantitative reverse transcription (qRT)-PCR. Figure S3. Coexpression relationships of genes preferentially expressed in root hairs. Figure S4. Root-hair phenotypes of mutants harboring defects in the expression of genes from the coexpression network. Figure S5. Validation of alternative-splicing features by quantitative reverse transcription (qRT)-PCR. Figure S6. Scheme for the identification and quantification of proteins. Figure S7. Distributions of molecular weight and isoelectric point of proteins. Figure S8. The interactome of root hairs.

Format: PDF Size: 1.6MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Dataset S1. Read numbers and RPKM values from the transcriptome analysis.

Format: XLSX Size: 3.3MB Download file

Open Data

Additional file 3:

Dataset S2. Differentially expressed genes.

Format: XLSX Size: 251KB Download file

Open Data

Additional file 4:

Tables S1-S7

Format: PDF Size: 768KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5:

Dataset S3. Splice junctions in EXP7 and non-GFP cells. GFP, green fluorescent protein.

Format: XLSX Size: 7.8MB Download file

Open Data

Additional file 6:

Dataset S4. Overview of alternative-splicing events in EXP7 and non-GFP cells. GFP, green fluorescent protein.

Format: XLSX Size: 2MB Download file

Open Data

Additional file 7:

Data set S5. Differential alternative-splicing events. Table S1. List of the root-related microarray experiments used for gene clustering. Table S2. Genes used for the search for expression signatures. Table S3. Post-translational modifications in the root protoplast proteome. Table S4. Root hair-specific proteins. Table S5. Differentially expressed proteins in EXP7 cells. Table S6. Confirmed interactions between root-hair proteins Table S7. Primers used for the validation of alternatively splicing features and differentially expressed genes.

Format: XLSX Size: 1.2MB Download file

Open Data