Method
A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases
- † Equal contributors
1 School of Biological Sciences, 50 Nanyang Avenue, Nanyang Technological University, Singapore 639798
2 Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
3 Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543
4 School of Applied Science, Temasek Polytechnic, 21 Tampines Avenue 1, Singapore 529757
5 NUS High School of Mathematics and Science, 20 Clementi Avenue 1, Singapore 129957
Genome Biology 2013, 14:R69 doi:10.1186/gb-2013-14-7-r69
Published: 1 July 2013Abstract
Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.
