Table 1 |
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Mutation frequencies induced by single TALEN pairs |
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Mutation frequency by T7E1 in individual embryos |
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TALEN pair |
Dosage |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
Mean ± SEM |
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|
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|
egfp5TAL |
12.5 pg |
15.07% |
8.67% |
25.40% |
16.99% |
16.40% |
20.68% |
16.57% |
18.42% |
13.85% |
9.88% |
16.2 ± 1.5% |
|
egfp3TAL |
12.5 pg |
25.57% |
14.43% |
46.31% |
20.71% |
30.76% |
24.42% |
21.81% |
24.62% |
11.36% |
34.57% |
25.5 ± 3.2% |
|
sqt5TAL |
25 pg |
8.09% |
7.17% |
7.32% |
4.18% |
11.85% |
8.47% |
6.81% |
12.83% |
10.42% |
13.84% |
9.1 ± 1.0% |
|
sqt3TAL |
25 pg |
9.77% |
5.91% |
4.89% |
1.90% |
3.46% |
2.00% |
4.37% |
4.44% |
5.48% |
3.22% |
4.5 ± 0.7% |
|
cyc5TAL |
12.5 pg |
17.53% |
46.86% |
25.65% |
24.72% |
40.45% |
27.34% |
44.29% |
32.57% |
25.27% |
34.58% |
31.9 ± 3.0% |
|
cyc3TAL |
12.5 pg |
43.76% |
58.13% |
59.00% |
52.41% |
57.46% |
70.59% |
65.23% |
40.71% |
41.66% |
50.57% |
54.0 ± 3.2% |
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For each single TALEN pair, ten injected embryos were assessed for mutation frequency by the T7E1 assay. SEM, standard error of the mean. |
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Lim et al. Genome Biology 2013 14:R69 doi:10.1186/gb-2013-14-7-r69 |
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