Table 1

Mutation frequencies induced by single TALEN pairs

Mutation frequency by T7E1 in individual embryos


TALEN pair

Dosage

1

2

3

4

5

6

7

8

9

10

Mean ± SEM


egfp5TAL

12.5 pg

15.07%

8.67%

25.40%

16.99%

16.40%

20.68%

16.57%

18.42%

13.85%

9.88%

16.2 ± 1.5%

egfp3TAL

12.5 pg

25.57%

14.43%

46.31%

20.71%

30.76%

24.42%

21.81%

24.62%

11.36%

34.57%

25.5 ± 3.2%

sqt5TAL

25 pg

8.09%

7.17%

7.32%

4.18%

11.85%

8.47%

6.81%

12.83%

10.42%

13.84%

9.1 ± 1.0%

sqt3TAL

25 pg

9.77%

5.91%

4.89%

1.90%

3.46%

2.00%

4.37%

4.44%

5.48%

3.22%

4.5 ± 0.7%

cyc5TAL

12.5 pg

17.53%

46.86%

25.65%

24.72%

40.45%

27.34%

44.29%

32.57%

25.27%

34.58%

31.9 ± 3.0%

cyc3TAL

12.5 pg

43.76%

58.13%

59.00%

52.41%

57.46%

70.59%

65.23%

40.71%

41.66%

50.57%

54.0 ± 3.2%


For each single TALEN pair, ten injected embryos were assessed for mutation frequency by the T7E1 assay. SEM, standard error of the mean.

Lim et al. Genome Biology 2013 14:R69   doi:10.1186/gb-2013-14-7-r69

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