Table 3

Frequency of cyclopia and mid-line defects in cyc and sqt nuclease-injected embryos

Targeting nuclease(s)

Wild type

Cyclopia and midline defects

Abnormal

Dead

Total (N)


6.25 pg cyc5TAL+cyc3TAL

176 (77.5%)

30 (13.2%)

8 (3.5%)

13 (5.8%)

227

12.5 pg cyc5TAL+cyc3TAL

134 (60.1%)

54 (24.2%)

17 (7.6%)

18 (8.1%)

223

25 pg cyc5TAL+cyc3TAL

11 (16.2%)

27 (39.7%)

27 (39.7%)

3 (4.4%)

68

25 pg sqtZFN2

62 (53.0%)

25 (21.4%)

18 (15.4%)

12 (10.2%)

117

50 pg sqtZFN2

32 (29.1%)

26 (23.6%)

37 (33.6%)

15 (13.6%)

110

25 pg sqt5TAL

22 (71.0%)

3 (9.7%)

0 (0.00%)

6 (19.3%)

31

50 pg sqt5TAL

43 (45.7%)

15 (16.0%)

18 (19.1%)

18 (19.1%)

94

25 pg sqt3TAL

18 (47.4%)

0 (0.00%)

5 (13.1%)

15 (39.5%)

38

50 pg sqt3TAL

29 (38.2%)

12 (15.8%)

15 (19.7%)

20 (26.3%)

76

25 pg sqt5TAL+sqtZFN2

22 (29.3%)

17 (22.7%)

19 (25.3%)

17 (22.7%)

75

25 pg sqt5TAL+sqt3TAL

30 (36.1%)

20 (24.1%)

15 (18.1%)

18 (21.7%)

83


Numbers were tabulated from at least two independent experiments.

Lim et al. Genome Biology 2013 14:R69   doi:10.1186/gb-2013-14-7-r69

Open Data