Table 3 |
|||||
|
Frequency of cyclopia and mid-line defects in cyc and sqt nuclease-injected embryos |
|||||
|
Targeting nuclease(s) |
Wild type |
Cyclopia and midline defects |
Abnormal |
Dead |
Total (N) |
|
|
|||||
|
6.25 pg cyc5TAL+cyc3TAL |
176 (77.5%) |
30 (13.2%) |
8 (3.5%) |
13 (5.8%) |
227 |
|
12.5 pg cyc5TAL+cyc3TAL |
134 (60.1%) |
54 (24.2%) |
17 (7.6%) |
18 (8.1%) |
223 |
|
25 pg cyc5TAL+cyc3TAL |
11 (16.2%) |
27 (39.7%) |
27 (39.7%) |
3 (4.4%) |
68 |
|
25 pg sqtZFN2 |
62 (53.0%) |
25 (21.4%) |
18 (15.4%) |
12 (10.2%) |
117 |
|
50 pg sqtZFN2 |
32 (29.1%) |
26 (23.6%) |
37 (33.6%) |
15 (13.6%) |
110 |
|
25 pg sqt5TAL |
22 (71.0%) |
3 (9.7%) |
0 (0.00%) |
6 (19.3%) |
31 |
|
50 pg sqt5TAL |
43 (45.7%) |
15 (16.0%) |
18 (19.1%) |
18 (19.1%) |
94 |
|
25 pg sqt3TAL |
18 (47.4%) |
0 (0.00%) |
5 (13.1%) |
15 (39.5%) |
38 |
|
50 pg sqt3TAL |
29 (38.2%) |
12 (15.8%) |
15 (19.7%) |
20 (26.3%) |
76 |
|
25 pg sqt5TAL+sqtZFN2 |
22 (29.3%) |
17 (22.7%) |
19 (25.3%) |
17 (22.7%) |
75 |
|
25 pg sqt5TAL+sqt3TAL |
30 (36.1%) |
20 (24.1%) |
15 (18.1%) |
18 (21.7%) |
83 |
|
|
|||||
|
Numbers were tabulated from at least two independent experiments. |
|||||
|
Lim et al. Genome Biology 2013 14:R69 doi:10.1186/gb-2013-14-7-r69 |
|||||
