Table 4 |
||
|
Germ-line transmission frequency of cyc and sqt nuclease-induced lesions in zebrafish |
||
|
Targeting nuclease(s) |
Number of F0 screened |
Number of mutant F0s |
|
|
||
|
sqt5TAL + sqt3TAL |
56 |
6 (whole locus deletions) |
|
sqt5TAL + sqtZFN2 |
28 |
2 (TSS deletions) |
|
sqtZFN1 |
92 |
1 (4 bp insertion) |
|
cyc5TAL + cyc3TAL |
36 |
10 (9 founders with TSS deletions, and 1 with a non-TSS 151 bp deletion and a deletion + inversion + insertion) |
|
|
||
|
For each founder (F0), at least 30 F1 embryos for sqt, or 22 F1 embryos for cyc were analyzed. |
||
|
Lim et al. Genome Biology 2013 14:R69 doi:10.1186/gb-2013-14-7-r69 |
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