Table 4

Germ-line transmission frequency of cyc and sqt nuclease-induced lesions in zebrafish

Targeting nuclease(s)

Number of F0 screened

Number of mutant F0s


sqt5TAL + sqt3TAL

56

6 (whole locus deletions)

sqt5TAL + sqtZFN2

28

2 (TSS deletions)

sqtZFN1

92

1 (4 bp insertion)

cyc5TAL + cyc3TAL

36

10 (9 founders with TSS deletions, and 1 with a non-TSS 151 bp deletion and a deletion + inversion + insertion)


For each founder (F0), at least 30 F1 embryos for sqt, or 22 F1 embryos for cyc were analyzed.

Lim et al. Genome Biology 2013 14:R69   doi:10.1186/gb-2013-14-7-r69

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