Table 5

Mutation frequency of double nuclease pairs versus homology directed repair

Clone size

Percentage of positive founders

Founders screened (n)

Source


cyc ΔTSS

4.5-22.5%

27.8%

36

This study

sqt ΔWL

3.3-9.5%

10.7%

56

This study

sqt ΔTSS

3.3-6.7%

7.1%

28

This study

apoea -477bp

2-11%

31.3%

16

Gupta et al. 2013 [13]

apoea -4.2kb

1-13%

29.4%

17

Gupta et al. 2013 [13]

th HDR

6.0-29.7%

1.5%

275

Zu et al. 2013 [29]

ponzr1 HDR

NA

~1.6%

186

Bedell et al. 2012 [30]

crhr2 HDR

NA

~13.8%

58

Bedell et al. 2012 [30]


Frequency of deletions in whole locus (ΔWL) and transcriptional start site (ΔTSS) for cyc and sqt compared to large deletions in apoea, and homology directed repair (HDR) at the th, ponzr and crhr2 loci are shown. NA, data not available.

Lim et al. Genome Biology 2013 14:R69   doi:10.1186/gb-2013-14-7-r69

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