Enrichment in binding sites defines an evolutionarily conserved signature of svb downstream genes. (a) Expression of svb mRNA determines the epidermal cells that form trichomes, visible on the larval cuticle. In situ hybridization shows mRNA expression of two svb downstream genes, shavenoid (sha/koj) and CG15589, in wild-type (wt; top) and svb mutant embryos (bottom). (b) Receiver operating characteristic curve showing significant enrichment in putative Svb binding sites (OvoQ6 position weight matrix) among the 39 Svb downstream genes (y-axis) compared to a randomized set of 1,000 Drosophila genes (x-axis) using cisTargetX. The blue curve shows the detection of Svb downstream genes, the red curve a random distribution, and the green curve shows a 2 sigma interval from random.
Menoret et al. Genome Biology 2013 14:R86 doi:10.1186/gb-2013-14-8-r86