5hmC distribution in pluripotent and differentiated cells. (A) Heatmaps of 5hmC occupancy on promoters and gene bodies in embryonic stem cells (ESCs), mouse embryonic fibroblasts (MEFs), and brain and liver tissue. Genes are ordered by the mRNA levels of each cell type obtained by RNA sequencing (RNA-Seq) analysis. (B) The 5hmC distribution profile in promoter regions and gene bodies of genes grouped into three equal sets by their mRNA expression level. (C) Heatmaps and distribution profiles of 5hmC in promoters and gene bodies of genes separated into two groups according to their expression level, as expressed only in liver (liver-specific) or expressed in in ESCs, MEFs, brain, and liver (housekeeping genes). (D) Scatter plot of 5hmC gene body density and expression level for each gene in ESCs, MEFs, brain, and liver.
Neri et al. Genome Biology 2013 14:R91 doi:10.1186/gb-2013-14-8-r91