Figure 5.

PRC2 interacts with Tet1 and is required for correct DNA hydroxymethylation. (A) Nuclear extracts of embryonic stem cells (ESCs) or mouse embryonic fibroblasts (MEFs) were immunoprecipitated with anti-IgG or anti-Tet1 antibodies. Western blotting analysis was performed using the antibodies indicated. For each input, 1% of nuclear extract was loaded. (B) Nuclear extracts of ESCs or MEFs were immunoprecipitated with anti-IgG or anti-Suz12 antibodies. Western blotting analysis was performed using the antibodies indicated. For each input, 2% of nuclear extract was loaded. (C) Western blotting analysis of extracts from the control (small hairpin green fluorescent protein; shGFP) or Suz12 knockdown (shSuz12) ESCs was performed using the antibodies indicated. (D) Dot-blot analysis and signal quantification of 5hmC and 5mC in DNA extracted from control or Suz12 knockdown ESCs. Single-stranded (ss)DNA was used as a loading control. The experiments were performed in triplicate.