Figure 6.

Polycomb repressive complex 2 (PRC2) is required for 5hmC deposition and Tet1 binding in Tet1-PRC2 co-bound regions. (A) Heatmaps and distribution profiles of 5hmC around the transcription start site (TSS) of genes rank-ordered by H3K27me3 levels in control or Suz12 knockdown embryonic stem cells (ESCs) See also Figure S3(C-E). (B) Sin3a, Tet1, H3K27me3, Suz12 occupancy and 5hmC occupancy in control and Suz12 knockdown cells on representative examples of bivalent (Kdr and Hoxa1) and K4-only (Cdc25a and Pou5f1) genes. Chromatin immunoprecipitation (ChIP) analysis of Tet1 binding of genomic regions (a to n) as indicated in panel (A), in control or Suz12 knockdown ESCs (*P < 0.01).