RNA-Seq evidence provides support for alternative isoforms. In this example from the bovine genome (Bos taurus) the RNA-Seq data was stored as a BAM file and dynamically uploaded. Individual aligned reads are shown in teal. The example highlights the importance of utilizing deep RNA sequencing for curation. Two different splice variants are visible: one variant is visible in the Dog Ensembl track and a different one is visible in the Mouse Ensembl track. The RNA-Seq data track clearly shows evidence that both variants are present in the bovine. Edge-matching (in red) highlights the concordance in exon boundaries between the different tracks.
Lee et al. Genome Biology 2013 14:R93 doi:10.1186/gb-2013-14-8-r93