Haplotyping with bacterial artificial chromosome (BAC) clones. (a) Constructing the BAC library. DNA was extracted from PGP1 (NA20431, Personal Genome Project) and BAC clone libraries with clone length (L= 140 kbp. (b) Forming pools of BAC clones. The number of pools (p) formed was 24, with each pool consisting of fourteen 16 × 24-well plates, so that there was a total of n = 5,376 clones per pool. (c) Sequencing and mapping each pool. Sequencing libraries were prepared for each pool with a read coverage of r = 6x. After sequencing, reads were mapped to hg19. (d) Reconstructing BAC clones. Clones were reconstructed from the mapped reads of each pool using coverage-based techniques (clones detected in region of chromosome 20).
Lo et al. Genome Biology 2013 14:R100 doi:10.1186/gb-2013-14-9-r100