Figure 2.

Haplotyping with bacterial artificial chromosome (BAC) clones. (a) Constructing the BAC library. DNA was extracted from PGP1 (NA20431, Personal Genome Project) and BAC clone libraries with clone length (L= 140 kbp. (b) Forming pools of BAC clones. The number of pools (p) formed was 24, with each pool consisting of fourteen 16 × 24-well plates, so that there was a total of n = 5,376 clones per pool. (c) Sequencing and mapping each pool. Sequencing libraries were prepared for each pool with a read coverage of r = 6x. After sequencing, reads were mapped to hg19. (d) Reconstructing BAC clones. Clones were reconstructed from the mapped reads of each pool using coverage-based techniques (clones detected in region of chromosome 20).