Table 2

Summary of Gene Ontology terms associated with positive and negative ageCGs affiliated with genes across tissuesa
GO termb Tissue Count P-value q-value Fold
Positive unique
GO:0030054 - cell junction Blood 42 5.2 × 10-10 7.0 × 10-7 3.1
GO:0048754 - branching morphogenesis of a tube Brain 18 1.6 × 10-7 3.0 × 10-4 5.0
GO:0048878 - chemical homeostasis Kidney 87 4.9 × 10-15 9.0 × 10-12 2.6
GO:0003713 - transcription coactivator activity Muscle 15 6.6 × 10-7 1.0 × 10-3 5.6
Positive two-way shared
GO:0000902 - cell morphogenesis Blood 37 2.3 × 10-14 4.0 × 10-11 4.9
GO:0003002 - regionalization Brain 41 5.3 × 10-15 9.6 × 10-12 4.6
GO:0000902 - cell morphogenesis Kidney 73 2.9 × 10-16 6.1 × 10-13 3.1
GO:0003712 - transcription cofactor activity Muscle 23 3.2 × 10-9 4.9 × 10-6 4.9
Positive three-way shared
GO:0007267 - cell-cell signaling Blood 56 8.9 × 10-28 1.6 × 10-24 6.5
GO:0007267 - cell-cell signaling Brain 64 4.3 × 10-21 7.8 × 10-18 4.2
GO:0030182 - neuron differentiation Kidney 117 4.5 × 10-37 8.2 × 10-34 4.1
GO:0016563 - transcription activator activity Muscle 29 5.0 × 10-10 7.5 × 10-7 4.2
Positive four-way shared
GO:0005886 - plasma membrane Blood 169 1.2 × 10-23 1.6 × 10-20 2.1
GO:0031226 - intrinsic to plasma membrane Brain 119 1.7 × 10-36 2.5 × 10-33 3.8
GO:0031226 - intrinsic to plasma membrane Kidney 209 1.3 × 10-60 1.9 × 10-57 3.7
GO:0006350 - transcription Muscle 102 1.5 × 10-18 2.6 × 10-15 2.6
Negative uniquec
GO:0006952 - defense response Kidney 58 5.8 × 10-30 1.0 × 10-26 6.8
GO:0005887 - integral to plasma membrane Kidney 68 2.0 × 10-22 2.7 × 10-19 4.0
GO:0006954 - inflammatory response Kidney 30 7.3 × 10-15 1.3 × 10-11 6.5
GO:0009611 - response to wounding Kidney 39 2.2 × 10-14 3.8 × 10-11 4.6
GO:0005886 - plasma membrane Kidney 138 9.1 × 10-14 1.2 × 10-10 1.8
GO:0006936 - muscle contraction Muscle 15 1.5 × 10-11 2.5 × 10-8 13.0
GO:0003012 - muscle system process Muscle 15 7.6 × 10-11 1.3 × 10-7 11.5
GO:0006941 - striated muscle contraction Muscle 10 1.3 × 10-9 2.2x10-6 21.3
GO:0015629 - actin cytoskeleton Muscle 15 3.6 × 10-6 4.7 × 10-3 4.8
GO:0003009 - skeletal muscle contraction Muscle 5 5.0 × 10-6 8.3 × 10-3 44.3
Negative two-way shared (kidney and blood only)d
GO:0006955 - immune response Kidney 66 2.6 × 10-32 4.5 × 10-29 6.3
GO:0031226 - intrinsic to plasma membrane Kidney 69 2.1 × 10-22 2.7 × 10-19 4.0
GO:0005576 - extracellular region Kidney 105 1.8 × 10-15 2.3 × 10-12 2.2
GO:0044459 - plasma membrane part Kidney 95 7.5 × 10-13 9.9 × 10-10 2.1
GO:0002684 - positive regulation of immune
system process Kidney 24 1.3 × 10-10 2.3 × 10-7 5.5

aDAVID v6.7 was used with all genes represented on the Methylation27 beadchip as background and gene lists associated for each tissue according to minimum age-associated P-value and positive or negative ageCGs. Enrichment tests were done independently for each tissue. Complete results are shown in supplemental data. All results shown are below q < 0.05 as generated by DAVID analysis [27,28].

bResults were first sorted by shared GO terms among tissues according to whether they overlapped among all four tissues (four-way), among three tissues (three-way), between two tissues (two-way), or unique in only one tissue. GO terms were then sorted by tissue and lowest q-values. The top GO term is shown for each tissue using this sorting scheme.

cThe top five genes affiliated with unique negative ageCGs in each tissue shown here do not include genes from brain because results did not meet q < 0.05for blood tissue see below.

dAll genes affiliated with negative ageCGs in blood were shared with kidney. Results are only shown here for kidney tissue.

Day et al.

Day et al. Genome Biology 2013 14:R102   doi:10.1186/gb-2013-14-9-r102

Open Data