PINT is required for Polycomb repressive complex 2 (PRC2) targeting of specific genes for repression. (A) Representation of the number of genes that are regulated by Pint in p53-p53LSL/LSL mouse embryonic fibroblasts (MEFs) (B > 3) (left) and/or reported as bound by Suz12 . The P-value represents the probability associated with the overlap between both gene sets. (B) Mean H3K27me3 ChIP-seq signal around the transcription start site (TSS) of genes regulated by Pint but not bound by Suz12 (blue)l genes bound by Suz12 but not regulated by Pint (red)l and genes regulated by Pint and bound by Suz12 (green) in mouse embryonic stem cells (mESCs) . (C) The most significant functions of genes regulated by Pint and bound by Suz12. (D,E) Relative (D) Suz12 or (E) H3K27me3 enrichment in promoter regions of H3K27me3-regulated genes  in doxorubicin (DOX)-treated p53-reconstituted p53LSL/LSL MEFs treated with Pint antisense oligonucleotides (ASOs) or control ASOs determined by chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR). Enrichment values are relative to IgG and the ASO control, and are the mean ± SD of three replicates. For each gene, asterisks indicate the significant difference between ASO Pint and ASO control: *P < 0.05; **P < 0.001(F) Relative cell numbers of control short hairpin RNA (shRNA) stable 3T3 MEFs transfected with the indicated ASOs or plasmids. (G) Relative cell numbers of Ezh2 shRNA stable 3T3 MEFs treated as in (F). Values are the mean ± SD of three replicates. *P < 0.05; **P < 0.001 relative to the control transfection.
Marín-Béjar et al. Genome Biology 2013 14:R104 doi:10.1186/gb-2013-14-9-r104