Figure 3.

Association of transcription factors with DNA methylation changes during moncoyte to OC differentiation. (A) Significant enrichment of predicted TF (TRANSFAC motif) in hypo-/hypermethylated CpG sites regions. A 500-bp window centered around the hypo-/hypermethylated CpG sites was tested. The name of the transcription factor binding motif, the P value and the TF family are provided. Below we show three of the motifs that have a higher representation in this analysis. (B) Diagrams showing the percentage of hypo-/hypermethylated CpGs with AP-1, NF-kB, and PU.1 binding sites relative to the total number of hypo-/hypermethylated CpGs. (C) Quantitative ChIP assays showing the binding of three selected transcription factors (p65 NF-kB subunit, Fos, and PU.1 to target genes selected by the presence of the putative binding motifs according to the TRANSFAC analysis). Samples were analyzed at 0 and 2 days after RANKL/M-CSF stimulation. We used Sat2 repeats and the TRDR1 MyoD1 promoter as negative control sequences.