Additional file 3.

(A) Scatterplots showing DNA methylation profiles of matching MO/OC pairs. Genes with significant differences (FC >2, FDR <0.05) in averaged results from three samples are highlighted in red (hypermethylated) or blue (hypomethylated). Three panels corresponding for each of the three individual comparisons of MO/OC pairs (D1, D2, and D3) are shown. (B) Bisulfite sequencing analysis of repetitive sequences performed on monocytes (day 0) and osteoclasts (day 21) from three different donors (donor A, donor B, and donor C), showing no relevant differences in the DNA methylation levels. (C) AUMA (amplification of unmethylated Alus) analysis of two independent monocyte-to-osteoclast differentiation experiments. Graphs correspond to the scanned intensities of the bands obtained with two different sets of primers. No significant differences are observed.

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de la Rica et al. Genome Biology 2013 14:R99   doi:10.1186/gb-2013-14-9-r99