Figure 1.

Control immunoprecipitations of PAR-CLIP gels show significant background RNA. Phosphorimages of SDS acrylamide gels following the PAR-CLIP protocol show radiolabeled RNPs from HEK293 cell lysates. The IP from each lysate contains four replicates. Brackets indicate extracted regions of the HuR RBP and controls using FLAG-GFP (tagged green fluorescent protein as G20, G35, G45) and no tagged protein.

Friedersdorf and Keene Genome Biology 2014 15:R2   doi:10.1186/gb-2014-15-1-r2
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