KLF1 activates while KLF3 represses chimeric transcripts from ORR1A0 LTRs in erythroid cells. RNA from Klf3+/+ (WT), Klf3+/− (HET), and Klf3−/− (KO) TER119+ E14.5 fetal liver cells (A, C, E) and from untreated and tamoxifen-treated KLF1-ER inducible B1.6 cells (B, D, F) was analyzed by quantitative real-time RT-PCR using forward primers which recognize the ORR1A0 exon and reverse primers specific for downstream exons of the Znrf2(A, B), Brca2(C, D) and Pqlc3(E, F) genes. All values have been normalized to 18S rRNA levels and WT (A, C, E) and untreated (B, D, F) samples have been set to 1.0. Error bars represent standard error of the mean and n = 3 for each genotype or condition. *, P <0.05 (Student’s two-tailed t-test) compared to both Klf3+/+ and Klf3+/−(A, C, E) and compared to untreated cells (B, D, F).
Mak et al. Genome Biology 2014 15:R58 doi:10.1186/gb-2014-15-4-r58