Additional file 8: Figure S6.

PU.2 opposes the transcriptional activity of PU.1. HEK293 cells were transfected with pLightSwitch Renilla luciferase reporter vector (promoter-less or containing the CLEC5A promoter). In (A), increasing amounts of pEF1α-Pu.1 have been co-transfected, while in (B), a steady amount of pEF1α-Pu.1 has been co-transfected together with increasing doses of pEF1α-Pu.2. In all experiments, pGL4.23[luc2/minP] Firefly luciferase vector was included as a transfection control and used for normalization. The means of triplicate experiments are shown and error bars represent standard error of the mean. *, P <0.05 (Student’s two-tailed t-test) compared to pLightSwitch-Clec5a wells transfected with 0 ng pEF1α-Pu.1 (A) or 0 ng pEF1α-Pu.2 (B).

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Mak et al. Genome Biology 2014 15:R58   doi:10.1186/gb-2014-15-4-r58