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Mapping-by-sequencing accelerates forward genetics in barley

Martin Mascher, Matthias Jost, Joel-Elias Kuon, Axel Himmelbach, Axel Aßfalg, Sebastian Beier, Uwe Scholz, Andreas Graner and Nils Stein*

Author Affiliations

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, Corrensstraße 3, 06466 Stadt Seeland, Germany

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Genome Biology 2014, 15:R78  doi:10.1186/gb-2014-15-6-r78

Published: 10 June 2014

Abstract

Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.