Genome Biology | Volume 2 Issue 2

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	Volume 2 Issue 2
	Comment
	Comment Homologuephobia Gregory A Petsko Genome Biology 2001, 2:comment1002-comment1002.2 (8 February 2001) Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Maybe I can't spell homolog, but I can at least use it correctly. Two sequences can be 43% identical or they can be 43% similar, but they can't be 43% homologous.
	Review
	Protein family review ClC chloride channels Joe Mindell, Merritt Maduke Genome Biology 2001, 2:reviews3003-reviews3003.6 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary ClC-type chloride-conducting ion channels are integral components of physiological processes throughout the biological world. These channels are fundamentally different in molecular architecture and mechanism from the better-known cation-conducting channels, as well as from other anion-conducting channels: as such they comprise a single protein family.
	Protein family review Higher plant glycosyltransferases Joe Ross, Yi Li, Eng-Kiat Lim, Dianna J Bowles Genome Biology 2001, 2:reviews3004-reviews3004.6 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Addition of a sugar molecule to a variety of substrates alters the activity, solubility and transport of the substrate. The UDP glycosyltransferases are one group of enzymes that carry out this reaction, and over 100 members of this protein family, all containing a 42 amino acid consensus sequence, have recently been shown to be encoded in the Arabidopsis genome.
	Minireview Protein profiling comes of age Ian M Tomlinson, Lucy J Holt Genome Biology 2001, 2:reviews1004-reviews1004.3 (31 January 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Ever since DNA microarrays were first applied to the quantitation of RNA levels, there has been considerable interest in generating a protein homolog that can be used to assay cellular protein expression. A recent paper describes the first microarray that can be used for such protein profiling.
	Minireview High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans Cornelia I Bargmann Genome Biology 2001, 2:reviews1005-reviews1005.3 (31 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Two recent chromosome-wide screens for phenotypes caused by RNA-mediated interference (RNAi) in Caenorhabditis elegans have increased our understanding of essential genes in nematodes. These papers represent a major advance in functional genomics.
	Minireview Control of eukaryotic transcription elongation Fred Winston Genome Biology 2001, 2:reviews1006-reviews1006.3 (31 January 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The Spt4, Spt5, and Spt6 proteins are conserved eukaryotic transcription-elongation factors. Recent studies have provided the first evidence that they are generally required in multicellular eukaryotes, including during development and for viral gene expression.
	Minireview Functional proteomics: large-scale analysis of protein kinase activity David S Lawrence Genome Biology 2001, 2:reviews1007-reviews1007.3 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Proteome-wide sampling of function can be used to shed light on complex biological systems. Protein microarrays have now been used to investigate the substrate specificities of essentially all the protein kinases encoded by the yeast genome.
	Opinion The complexity of simplicity Scott N Peterson, Claire M Fraser Genome Biology 2001, 2:comment2002-comment2002.8 (8 February 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary What is the minimum number of genes or functions necessary to support cellular life? The concept of a 'minimal genome' has become popular, but is it a useful concept, and if so, what might a minimal genome encode? We argue that the concept may be useful, even though the goal of defining a general minimal genome may never be attained.
	Report
	Web report Statistical resources on the web I King Jordan Genome Biology 2001, 2:reports2002 (1 February 2001) Abstract \| Full text
	Paper report Systematic RNAi in C. elegans Rachel Brem Genome Biology 2001, 2:reports0002 (31 January 2001) Abstract \| Full text \| Editor’s summary Caenorhabditis elegans populations in which each gene on chromosome I is blocked in turn by RNAi have been grown and phenotypically screened.
	Paper report Key players in yeast sporulation Rachel Brem Genome Biology 2001, 2:reports0003 (31 January 2001) Abstract \| Full text \| Editor’s summary Expression arrays have been used to detect mRNAs regulated during sporulation in two strains of Saccharomyces cerevisiae.
	Paper report Eukaryotic transcription factors Thomas Eulgem Genome Biology 2001, 2:reports0004 (8 February 2001) Abstract \| Full text
	Paper report Clock-controlled genes in Arabidopsis Thomas Eulgem Genome Biology 2001, 2:reports0005 (8 February 2001) Abstract \| Full text \| Editor’s summary Large-scale expression profiling has revealed distinct clusters of circadian clock-regulated genes and identified a phase-specific cis-regulatory element.
	Paper report Retroviruses from retrotransposons James Cotton Genome Biology 2001, 2:reports0006 (8 February 2001) Abstract \| Full text
	Meeting report Follow that plant! Pablo D Rabinowicz, Mary E Byrne Genome Biology 2001, 2:reports4002-reports4002.3 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A report on the talks presented at the Cold Spring Harbor 2000 Meeting on Arabidopsis Genomics, New York, 7-10 December, 2000.
	Meeting report Making sense of microarrays James N Siedow Genome Biology 2001, 2:reports4003-reports4003.2 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A report on the 'Critical Assessment of Microarray Data Analysis' (CAMDA 2000) meeting, Durham, North Carolina, USA, December 18-19,2000.
	Meeting report Non-classical immunology Rachel L Allen Genome Biology 2001, 2:reports4004-reports4004.4 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A report on the British Society for Immunology Annual Congress, Harrogate, UK, 5-8 December 2000.
	Research news Array-of-arrays Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010201-01 (1 February 2001) Abstract \| Full text \| Editor’s summary Highly parallel microarray screening allows the rapid identification of marker genes for epithelial ovarian cancer.
	Research news Syngenta claims ownership of rice - but will give data away Robert Walgate Genome Biology 2001, 2:spotlight-20010201-02 (1 February 2001) Abstract \| Full text \| Editor’s summary The company that sequenced the rice genome says it will retain commercial rights, but will give data to academics - and useful technologies to farmers in the developing world.
	Research news Gut genomics Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010206-02 (6 February 2001) Abstract \| Full text \| Editor’s summary Genomics analysis reveals molecular details of the transcriptional response to colonization of the intestine by resident bacteria.
	Research news Putting an end to chromosome stability Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010206-01 (6 February 2001) Abstract \| Full text \| Editor’s summary Telomere repeat sequences induce chromosomal instability and gene rearrangements when inserted inside the chromosome.
	Research news Nuclear reprogramming in cloned cows Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010207-01 (7 February 2001) Abstract \| Full text \| Editor’s summary Donor nuclei from somatic cells are reprogrammed in cloned bovine embryos to restore normal telomere length.
	Research news An encyclopaedia of mouse genes Kenneth Lee Genome Biology 2001, 2:spotlight-20010208-02 (8 February 2001) Abstract \| Full text \| Editor’s summary An international consortium of scientists aiming to sequence every transcript encoded by the mouse genome has analysed 21,076 so far.
	Research news Speciation induced by a bacterial symbiont? Kenneth Lee Genome Biology 2001, 2:spotlight-20010208-01 (8 February 2001) Abstract \| Full text \| Editor’s summary The cytoplasmic symbiotic bacteria Wolbachia could induce host speciation in insects.
	Research news The genome has landed - let the debate begin Pete Moore Genome Biology 2001, 2:spotlight-20010212-01 (12 February 2001) Abstract \| Full text \| Editor’s summary The carefully choreographed publication in Science and Nature of papers laying bare the human genome brings the first chapter of genomic research towards its completion.
	Research news The Celera paper: sequencing by random shotgun cloning Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010213-01 (13 February 2001) Abstract \| Full text \| Editor’s summary The private biotechnology company Celera Genomics has sequenced the human genome by a high-throughput shotgun sequencing approach.
	Research news The Human Genome Consortium paper: sequencing by collaborative mapping Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010213-02 (13 February 2001) Abstract \| Full text \| Editor’s summary An international Consortium has achieved the first draft of the human genome sequence by a collaborative, map-based approach.
	Research news Maternal mutation in trans Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010215-01 (15 February 2001) Abstract \| Full text \| Editor’s summary Experiments with irradiated mice show that damage to DNA in the paternal genome is hazardous for the maternally derived genome.
	Research news Mosquito MITEs Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010215-02 (15 February 2001) Abstract \| Full text \| Editor’s summary Bioinformatics tool identifies families of transposable elements in the malaria mosquito.
	Research news A single gene drives endocrine pancreatic development Kenneth Lee Genome Biology 2001, 2:spotlight-20010216-01 (16 February 2001) Abstract \| Full text \| Editor’s summary Experiments using chick embryos show that a single gene can drive the development of hormone-secreting alpha and beta pancreatic islet cells.
	Research news A pathway leading to activation of BRCA1 Kenneth Lee Genome Biology 2001, 2:spotlight-20010219-01 (19 February 2001) Abstract \| Full text \| Editor’s summary A protein involved in Fanconi anaemia has been identified; it also interacts with the breast cancer susceptibility gene BRCA1.
	Research news Restriction enzyme scissor cut Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010219-02 (19 February 2001) Abstract \| Full text \| Editor’s summary The crystal structure of the free BglII restriction enzyme reveals an unusual scissor-like motion that allows DNA entry.
	Research news Autoimmune diseases get the NOD Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010219-03 (19 February 2001) Abstract \| Full text \| Editor’s summary Observations in a mouse model of diabetes have led to the discovery of a human susceptibility locus.
	Research news Cloning big sheep Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010221-02 (21 February 2001) Abstract \| Full text \| Editor’s summary Epigenetic alteration of imprinted gene expression accounts for fetal overgrowth of cloned sheep.
	Research news Mitochondrial mutation associated with hearing loss Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010221-01 (21 February 2001) Abstract \| Full text \| Editor’s summary An unprecedented interaction between nuclear and mitochondrial genomes affects hearing loss in mice.
	Research news Neurogenin works in two ways Tudor Toma Genome Biology 2001, 2:spotlight-20010222-01 (22 February 2001) Abstract \| Full text \| Editor’s summary The transcription factor neurogenin induces neurogenesis and inhibits the differentiation of neural stem cells into astrocytes using distinct mechanisms.
	Research news The joint gene Tudor Toma Genome Biology 2001, 2:spotlight-20010222-02 (22 February 2001) Abstract \| Full text \| Editor’s summary Wnt-14, a member of the Wnt gene family, actively directs prechondrogenic cells into the joint-forming pathway and has a role in initiating synovial joint formation.
	Research news Decay in the leprosy genome Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010222-03 (22 February 2001) Abstract \| Full text \| Editor’s summary The genome of the pathogen Mycobacterium leprae reveals striking evidence of reductive evolution.
	Research news Editing the immune system Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010223-02 (23 February 2001) Abstract \| Full text \| Editor’s summary Experiments with transgenic mice demonstrate that receptor editing in B cells plays a major role in determining the antibody repertoire.
	Research news "Massive decay" in leprosy genome - massive for research too? Robert Walgate Genome Biology 2001, 2:spotlight-20010223-01 (23 February 2001) Abstract \| Full text \| Editor’s summary The genome of Mycobacterium leprae has only half the active genes of its close relative M. tuberculosis.
	Research news Using both strands Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010226-01 (26 February 2001) Abstract \| Full text \| Editor’s summary Two mRNA precursors generated from both complimentary DNA strands of a gene appear to be @trans#-spliced to form a single message.
	Research news Automatable SNP assay Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010227-01 (27 February 2001) Abstract \| Full text \| Editor’s summary A fluorescence-based assay for single-nucleotide polymorphisms that can be easily automated has been developed.
	Research news BRCA cancer mutations influence gene expression Tudor Toma Genome Biology 2001, 2:spotlight-20010228-02 (28 February 2001) Abstract \| Full text \| Editor’s summary Breast cancers with mutations in either BRCA1 or BRCA2 express different groups of genes, suggesting that a heritable mutation influences the gene-expression profile of the cancer.
	Research news Early breast cancer detection Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010228-03 (28 February 2001) Abstract \| Full text \| Editor’s summary Microarrays have been used to screen peripheral blood for disseminated breast tumour cells.
	Research news Gene for DiGeorge syndrome Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010228-04 (28 February 2001) Abstract \| Full text \| Editor’s summary Haploinsufficiency of the murine TBX1 gene causes cardiovascular defects similar to features of human DiGeorge syndrome.
	Research news e-publishing - Paris, profit and potential Pete Moore Genome Biology 2001, 2:spotlight-20010228-01 (28 February 2001) Abstract \| Full text \| Editor’s summary A UNESCO meeting in Paris last week discussed the potential of electronic publishing in science. There have been some strong movements in biomedical publishing in the past five years but nothing like the seismic shift that's needed, concludes physicist Paul Ginsparg.
	Research
	Research Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions Brian B Haab, Maitreya J Dunham, Patrick O Brown Genome Biology 2001, 2:research0004-research0004.13 (22 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary A method is described for printing protein microarrays and using them in a comparative fluorescence assay to measure the abundance of specific proteins in complex solutions. Antigens or antibodies were spotted onto the arrays, and accurate measurements of their cognate ligands were made at concentrations of 1.6 μg/ml or below. Some antibody-antigen pairs allowed detection of the cognate ligands at absolute concentrations below 1 ng/ml and partial concentrations of less than 1 part in 10⁶, sensitivities sufficient for measurement of many clinically important proteins in patient blood samples.
	Research Both subtelomeric regions are required and sufficient for specific DNA fragmentation during macronuclear development in Stylonychia lemnae Franziska Jönsson, Günther Steinbrück, Hans J Lipps Genome Biology 2001, 2:research0005-research0005.11 (25 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Programmed RNA-reorganization and DNA-elimination events take place frequently during cellular differentiation. In the ciliate Stylonychia lemnae cis-acting sequences required for these events are localized in the 3'- and the 5' subteleomeric regions of the macronuclear precursor sequence, and an interaction between the two regions seems to occur.
	Research Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates Eric J Devor Genome Biology 2001, 2:research0006-research0006.5 (29 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary 'Molecular beacon' assays show that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is found only in Primates. Its presence in Old World and New World species but not in prosimians suggests that SHMT-ps1 originated close to the origin of the Anthropoidea, some 40 to 50 million years ago.

	Volume 2 Issue 2
	Comment
	Comment Homologuephobia Gregory A Petsko Genome Biology 2001, 2:comment1002-comment1002.2 (8 February 2001) Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Maybe I can't spell homolog, but I can at least use it correctly. Two sequences can be 43% identical or they can be 43% similar, but they can't be 43% homologous.
	Review
	Protein family review ClC chloride channels Joe Mindell, Merritt Maduke Genome Biology 2001, 2:reviews3003-reviews3003.6 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary ClC-type chloride-conducting ion channels are integral components of physiological processes throughout the biological world. These channels are fundamentally different in molecular architecture and mechanism from the better-known cation-conducting channels, as well as from other anion-conducting channels: as such they comprise a single protein family.
	Protein family review Higher plant glycosyltransferases Joe Ross, Yi Li, Eng-Kiat Lim, Dianna J Bowles Genome Biology 2001, 2:reviews3004-reviews3004.6 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Addition of a sugar molecule to a variety of substrates alters the activity, solubility and transport of the substrate. The UDP glycosyltransferases are one group of enzymes that carry out this reaction, and over 100 members of this protein family, all containing a 42 amino acid consensus sequence, have recently been shown to be encoded in the Arabidopsis genome.
	Minireview Protein profiling comes of age Ian M Tomlinson, Lucy J Holt Genome Biology 2001, 2:reviews1004-reviews1004.3 (31 January 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Ever since DNA microarrays were first applied to the quantitation of RNA levels, there has been considerable interest in generating a protein homolog that can be used to assay cellular protein expression. A recent paper describes the first microarray that can be used for such protein profiling.
	Minireview High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans Cornelia I Bargmann Genome Biology 2001, 2:reviews1005-reviews1005.3 (31 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Two recent chromosome-wide screens for phenotypes caused by RNA-mediated interference (RNAi) in Caenorhabditis elegans have increased our understanding of essential genes in nematodes. These papers represent a major advance in functional genomics.
	Minireview Control of eukaryotic transcription elongation Fred Winston Genome Biology 2001, 2:reviews1006-reviews1006.3 (31 January 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The Spt4, Spt5, and Spt6 proteins are conserved eukaryotic transcription-elongation factors. Recent studies have provided the first evidence that they are generally required in multicellular eukaryotes, including during development and for viral gene expression.
	Minireview Functional proteomics: large-scale analysis of protein kinase activity David S Lawrence Genome Biology 2001, 2:reviews1007-reviews1007.3 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Proteome-wide sampling of function can be used to shed light on complex biological systems. Protein microarrays have now been used to investigate the substrate specificities of essentially all the protein kinases encoded by the yeast genome.
	Opinion The complexity of simplicity Scott N Peterson, Claire M Fraser Genome Biology 2001, 2:comment2002-comment2002.8 (8 February 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary What is the minimum number of genes or functions necessary to support cellular life? The concept of a 'minimal genome' has become popular, but is it a useful concept, and if so, what might a minimal genome encode? We argue that the concept may be useful, even though the goal of defining a general minimal genome may never be attained.
	Report
	Web report Statistical resources on the web I King Jordan Genome Biology 2001, 2:reports2002 (1 February 2001) Abstract \| Full text
	Paper report Systematic RNAi in C. elegans Rachel Brem Genome Biology 2001, 2:reports0002 (31 January 2001) Abstract \| Full text \| Editor’s summary Caenorhabditis elegans populations in which each gene on chromosome I is blocked in turn by RNAi have been grown and phenotypically screened.
	Paper report Key players in yeast sporulation Rachel Brem Genome Biology 2001, 2:reports0003 (31 January 2001) Abstract \| Full text \| Editor’s summary Expression arrays have been used to detect mRNAs regulated during sporulation in two strains of Saccharomyces cerevisiae.
	Paper report Eukaryotic transcription factors Thomas Eulgem Genome Biology 2001, 2:reports0004 (8 February 2001) Abstract \| Full text
	Paper report Clock-controlled genes in Arabidopsis Thomas Eulgem Genome Biology 2001, 2:reports0005 (8 February 2001) Abstract \| Full text \| Editor’s summary Large-scale expression profiling has revealed distinct clusters of circadian clock-regulated genes and identified a phase-specific cis-regulatory element.
	Paper report Retroviruses from retrotransposons James Cotton Genome Biology 2001, 2:reports0006 (8 February 2001) Abstract \| Full text
	Meeting report Follow that plant! Pablo D Rabinowicz, Mary E Byrne Genome Biology 2001, 2:reports4002-reports4002.3 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A report on the talks presented at the Cold Spring Harbor 2000 Meeting on Arabidopsis Genomics, New York, 7-10 December, 2000.
	Meeting report Making sense of microarrays James N Siedow Genome Biology 2001, 2:reports4003-reports4003.2 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A report on the 'Critical Assessment of Microarray Data Analysis' (CAMDA 2000) meeting, Durham, North Carolina, USA, December 18-19,2000.
	Meeting report Non-classical immunology Rachel L Allen Genome Biology 2001, 2:reports4004-reports4004.4 (7 February 2001) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A report on the British Society for Immunology Annual Congress, Harrogate, UK, 5-8 December 2000.
	Research news Array-of-arrays Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010201-01 (1 February 2001) Abstract \| Full text \| Editor’s summary Highly parallel microarray screening allows the rapid identification of marker genes for epithelial ovarian cancer.
	Research news Syngenta claims ownership of rice - but will give data away Robert Walgate Genome Biology 2001, 2:spotlight-20010201-02 (1 February 2001) Abstract \| Full text \| Editor’s summary The company that sequenced the rice genome says it will retain commercial rights, but will give data to academics - and useful technologies to farmers in the developing world.
	Research news Gut genomics Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010206-02 (6 February 2001) Abstract \| Full text \| Editor’s summary Genomics analysis reveals molecular details of the transcriptional response to colonization of the intestine by resident bacteria.
	Research news Putting an end to chromosome stability Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010206-01 (6 February 2001) Abstract \| Full text \| Editor’s summary Telomere repeat sequences induce chromosomal instability and gene rearrangements when inserted inside the chromosome.
	Research news Nuclear reprogramming in cloned cows Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010207-01 (7 February 2001) Abstract \| Full text \| Editor’s summary Donor nuclei from somatic cells are reprogrammed in cloned bovine embryos to restore normal telomere length.
	Research news An encyclopaedia of mouse genes Kenneth Lee Genome Biology 2001, 2:spotlight-20010208-02 (8 February 2001) Abstract \| Full text \| Editor’s summary An international consortium of scientists aiming to sequence every transcript encoded by the mouse genome has analysed 21,076 so far.
	Research news Speciation induced by a bacterial symbiont? Kenneth Lee Genome Biology 2001, 2:spotlight-20010208-01 (8 February 2001) Abstract \| Full text \| Editor’s summary The cytoplasmic symbiotic bacteria Wolbachia could induce host speciation in insects.
	Research news The genome has landed - let the debate begin Pete Moore Genome Biology 2001, 2:spotlight-20010212-01 (12 February 2001) Abstract \| Full text \| Editor’s summary The carefully choreographed publication in Science and Nature of papers laying bare the human genome brings the first chapter of genomic research towards its completion.
	Research news The Celera paper: sequencing by random shotgun cloning Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010213-01 (13 February 2001) Abstract \| Full text \| Editor’s summary The private biotechnology company Celera Genomics has sequenced the human genome by a high-throughput shotgun sequencing approach.
	Research news The Human Genome Consortium paper: sequencing by collaborative mapping Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010213-02 (13 February 2001) Abstract \| Full text \| Editor’s summary An international Consortium has achieved the first draft of the human genome sequence by a collaborative, map-based approach.
	Research news Maternal mutation in trans Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010215-01 (15 February 2001) Abstract \| Full text \| Editor’s summary Experiments with irradiated mice show that damage to DNA in the paternal genome is hazardous for the maternally derived genome.
	Research news Mosquito MITEs Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010215-02 (15 February 2001) Abstract \| Full text \| Editor’s summary Bioinformatics tool identifies families of transposable elements in the malaria mosquito.
	Research news A single gene drives endocrine pancreatic development Kenneth Lee Genome Biology 2001, 2:spotlight-20010216-01 (16 February 2001) Abstract \| Full text \| Editor’s summary Experiments using chick embryos show that a single gene can drive the development of hormone-secreting alpha and beta pancreatic islet cells.
	Research news A pathway leading to activation of BRCA1 Kenneth Lee Genome Biology 2001, 2:spotlight-20010219-01 (19 February 2001) Abstract \| Full text \| Editor’s summary A protein involved in Fanconi anaemia has been identified; it also interacts with the breast cancer susceptibility gene BRCA1.
	Research news Restriction enzyme scissor cut Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010219-02 (19 February 2001) Abstract \| Full text \| Editor’s summary The crystal structure of the free BglII restriction enzyme reveals an unusual scissor-like motion that allows DNA entry.
	Research news Autoimmune diseases get the NOD Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010219-03 (19 February 2001) Abstract \| Full text \| Editor’s summary Observations in a mouse model of diabetes have led to the discovery of a human susceptibility locus.
	Research news Cloning big sheep Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010221-02 (21 February 2001) Abstract \| Full text \| Editor’s summary Epigenetic alteration of imprinted gene expression accounts for fetal overgrowth of cloned sheep.
	Research news Mitochondrial mutation associated with hearing loss Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010221-01 (21 February 2001) Abstract \| Full text \| Editor’s summary An unprecedented interaction between nuclear and mitochondrial genomes affects hearing loss in mice.
	Research news Neurogenin works in two ways Tudor Toma Genome Biology 2001, 2:spotlight-20010222-01 (22 February 2001) Abstract \| Full text \| Editor’s summary The transcription factor neurogenin induces neurogenesis and inhibits the differentiation of neural stem cells into astrocytes using distinct mechanisms.
	Research news The joint gene Tudor Toma Genome Biology 2001, 2:spotlight-20010222-02 (22 February 2001) Abstract \| Full text \| Editor’s summary Wnt-14, a member of the Wnt gene family, actively directs prechondrogenic cells into the joint-forming pathway and has a role in initiating synovial joint formation.
	Research news Decay in the leprosy genome Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010222-03 (22 February 2001) Abstract \| Full text \| Editor’s summary The genome of the pathogen Mycobacterium leprae reveals striking evidence of reductive evolution.
	Research news Editing the immune system Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010223-02 (23 February 2001) Abstract \| Full text \| Editor’s summary Experiments with transgenic mice demonstrate that receptor editing in B cells plays a major role in determining the antibody repertoire.
	Research news "Massive decay" in leprosy genome - massive for research too? Robert Walgate Genome Biology 2001, 2:spotlight-20010223-01 (23 February 2001) Abstract \| Full text \| Editor’s summary The genome of Mycobacterium leprae has only half the active genes of its close relative M. tuberculosis.
	Research news Using both strands Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010226-01 (26 February 2001) Abstract \| Full text \| Editor’s summary Two mRNA precursors generated from both complimentary DNA strands of a gene appear to be @trans#-spliced to form a single message.
	Research news Automatable SNP assay Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010227-01 (27 February 2001) Abstract \| Full text \| Editor’s summary A fluorescence-based assay for single-nucleotide polymorphisms that can be easily automated has been developed.
	Research news BRCA cancer mutations influence gene expression Tudor Toma Genome Biology 2001, 2:spotlight-20010228-02 (28 February 2001) Abstract \| Full text \| Editor’s summary Breast cancers with mutations in either BRCA1 or BRCA2 express different groups of genes, suggesting that a heritable mutation influences the gene-expression profile of the cancer.
	Research news Early breast cancer detection Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010228-03 (28 February 2001) Abstract \| Full text \| Editor’s summary Microarrays have been used to screen peripheral blood for disseminated breast tumour cells.
	Research news Gene for DiGeorge syndrome Jonathan B Weitzman Genome Biology 2001, 2:spotlight-20010228-04 (28 February 2001) Abstract \| Full text \| Editor’s summary Haploinsufficiency of the murine TBX1 gene causes cardiovascular defects similar to features of human DiGeorge syndrome.
	Research news e-publishing - Paris, profit and potential Pete Moore Genome Biology 2001, 2:spotlight-20010228-01 (28 February 2001) Abstract \| Full text \| Editor’s summary A UNESCO meeting in Paris last week discussed the potential of electronic publishing in science. There have been some strong movements in biomedical publishing in the past five years but nothing like the seismic shift that's needed, concludes physicist Paul Ginsparg.
	Research
	Research Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions Brian B Haab, Maitreya J Dunham, Patrick O Brown Genome Biology 2001, 2:research0004-research0004.13 (22 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary A method is described for printing protein microarrays and using them in a comparative fluorescence assay to measure the abundance of specific proteins in complex solutions. Antigens or antibodies were spotted onto the arrays, and accurate measurements of their cognate ligands were made at concentrations of 1.6 μg/ml or below. Some antibody-antigen pairs allowed detection of the cognate ligands at absolute concentrations below 1 ng/ml and partial concentrations of less than 1 part in 10⁶, sensitivities sufficient for measurement of many clinically important proteins in patient blood samples.
	Research Both subtelomeric regions are required and sufficient for specific DNA fragmentation during macronuclear development in Stylonychia lemnae Franziska Jönsson, Günther Steinbrück, Hans J Lipps Genome Biology 2001, 2:research0005-research0005.11 (25 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Programmed RNA-reorganization and DNA-elimination events take place frequently during cellular differentiation. In the ciliate Stylonychia lemnae cis-acting sequences required for these events are localized in the 3'- and the 5' subteleomeric regions of the macronuclear precursor sequence, and an interaction between the two regions seems to occur.
	Research Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates Eric J Devor Genome Biology 2001, 2:research0006-research0006.5 (29 January 2001) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary 'Molecular beacon' assays show that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is found only in Primates. Its presence in Old World and New World species but not in prosimians suggests that SHMT-ps1 originated close to the origin of the Anthropoidea, some 40 to 50 million years ago.

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