MADS-complexes regulate transcriptome dynamics during pollen maturation

Wim Verelst¹ , David Twell² , Stefan de Folter³^,4 , Richard Immink² , Heinz Saedler¹ and Thomas Münster¹

¹Department of Molecular Plant Genetics, Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg, 50829 Cologne, Germany

²Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK

³Business Unit Bioscience, Plant Research International, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands

⁴National Laboratory of Genomics for Biodiversity (Langebio), Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), 36500 Irapuato, Guanajuato, Mexico

author email corresponding author email

Genome Biology 2007, 8:R249doi:10.1186/gb-2007-8-11-r249


Published:	22 November 2007

Subject areas: Plant biology, Genome studies, Molecular biology

Additional files

Additional data file 1:

As described in Materials and methods, we examined various functional and morphological aspects of WT and agl65/66/104 triple mutant pollen grains. Apart from (a) a nearly complete block in in vitro pollen germination, triple mutant pollen was indistinguishable from the WT, with respect to (b) in vivo germination and pollen tube growth, (c) viability and membrane integrity, (d) nuclear number and organization, (e) surface appearance, (f) ultrastructure, (g) cellulose, (h) callose, and (i,j) pectin.

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Additional data file 2:

This Excel file contains relevant information about the genes consistently affected in triple mutant pollen, including all related information we used in figures and calculations. Separate legends are included in each worksheet.

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Additional data file 3:

In this presentation based on MapMan [33], part of the synthesis pathway for cell wall components is shown. Blue boxes indicate that a gene responsible for a reaction is upregulated in triple mutant pollen, and red boxes indicate downregulated genes. For each gene the expression profile throughout pollen development is added (based on Honys and Twell [11]). Almost without exception immature-pollen genes are upregulated in triple mutant pollen, while the few mature pollen genes in this pathway are downregulated.

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Additional data file 4:

Based on the general trend of their expression in the different AtMIKC* single and double mutants - which reflects the impact of loss of the individual AtMIKC* complexes on their expression level - we defined five categories of AtMIKC*-regulated genes.

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Additional data file 5:

For a selection of the genes affected in agl65/66/104 triple mutant pollen (identified by transcriptome analysis) we verified the differential expression in WT and triple mutant pollen by RT-PCR, using RNA from independently harvested pollen samples.

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Additional data file 6:

In a yeast-three-hybrid experiment the previously identified AtMIKC* heterodimeric complexes [28] were tested for ternary interaction with the individual AtMIKC* proteins, and with AGL18 and AGL29. At least nine AtMIKC* higher-order complexes could be reliably detected in yeast. AGL18 and AGL29 did not interact with AtMIKC* proteins.

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