Genome Biology
Volume 9
Issue 5

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Oh Y
Donofrio N
Pan H
Coughlan S
Brown DE
Meng S
Mitchell T
Dean RA

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Oh Y
Donofrio N
Pan H
Coughlan S
Brown DE
Meng S
Mitchell T
Dean RA
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Research

Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae

Yeonyee Oh¹ , Nicole Donofrio¹^,3 , Huaqin Pan¹^,4 , Sean Coughlan² , Douglas E Brown¹ , Shaowu Meng¹ , Thomas Mitchell¹^,5 and Ralph A Dean¹

¹North Carolina State University, Center for Integrated Fungal Research, Raleigh, NC 27695-7251, USA

²Agilent Technologies, Little Falls, DE 19808-1644, USA

³Current address: University of Delaware, Department of Plant and Soil Science, Newark, DE 19716, USA

⁴Current address: RTI international, Research Triangle Park, NC 27709-2194, USA

⁵Current address: Ohio State University, Department of Plant Pathology, Columbus, OH 43210, USA

author email corresponding author email

Genome Biology 2008, 9:R85doi:10.1186/gb-2008-9-5-r85


Published:	20 May 2008

Subject areas: Cell biology, Development, Genome studies, Microbiology and parasitology

Additional files

Additional data file 1:

GO categorization (biological process) of differentially expressed genes during spore germination.

Format: XLS Size: 306KB Download file

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Additional data file 2:

Primer sequences for RT-PCR and qRT-PCR.

Format: XLS Size: 19KB Download file

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Additional data file 3:

Categorization of appressorium consensus genes with known function.

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Additional data file 4:

Differential expression of the putative melanin biosynthesis gene cluster during appressorium formation.

Format: DOC Size: 200KB Download file

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Additional data file 5:

Adaptamer mediated PCR strategy for targeted gene deletion.

Format: DOC Size: 171KB Download file

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Additional data file 6:

Primer sequences for gene specific replacement cassette construction.

Format: XLS Size: 33KB Download file

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Additional data file 7:

Confirmation of target gene replacement by PCR.

Format: DOC Size: 223KB Download file

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