Figure 3.

RT-PCR and qRT-PCR analysis of gene expression. (a) RT-PCR using RNA isolated from spores germinated on the hydrophobic (Pho12) and hydrophilic (Phil12) surfaces of GelBond after 12 h incubation compared to expression fold change (FC) derived from microarray data from the same time point. (b) qRT-PCR analysis of MPG1 and PTH11 using RNA from appressoria induced by cAMP (+cAMP) and germinating spores (-cAMP) after 9 h incubation on the hydrophilic surface of GelBond. Gene expression fold changes for MPG1 and PTH11 were 0.2 and 0.2, respectively, in our cAMP microarray study.