gb-2004-5-4-p8 GBJ Deposited research article <p>Characterization of Indian cattle breeds, Ongole and Deoni (<it>Bos indicus</it>) using microsatellite markers</p> Metta Muralidhar Kanginakudru Sriramana Gudiseva Narasimharao Nagaraju Javaregowda jnagaraju@cdfd.org.in

Department of Animal Genetics and Breeding, College of Veterinary Science, A NG R Agricultural University, Hyderabad 500 030, India

Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500 076, India

 Genome Biology 1465-6906 2004 5 4 P8 http://genomebiology.com/2004/5/4/P8 This was the first version of this article to be made available publicly. A peer-reviewed and modified version is now available in full at http://www.biomedcentral.com/1471-2156/5/16 10.1186/gb-2004-5-4-p8 1 3 2004 4 3 2004 2003 BioMed Central Ltd

Two Indian cattle breeds, Ongole and Deoni, were characterized using microsatellite markers, revealing six Ongole and three Deoni specific alleles.

Abstract

Molecular characterization of cattle breeds is important for the prevention of germplasm erosion by cross breeding. The present study was carried out to characterize two Indian cattle breeds, Ongole and Deoni using microsatellite markers. Using 5 di-and 5 tri- nucleotide repeat loci, 17 Ongole and 13 Deoni unrelated individuals were studied. Of the ten loci, eight revealed polymorphism in both the breeds. The di-nucleotide repeats loci were found to be more polymorphic (100%) than tri-nucleotide repeat loci (60%). A total of 39 polymorphic alleles were obtained at 4.5 alleles per locus in Ongole and 4.1 in Deoni. The average expected heterozygosity was 0.46 (

    +
0.1) and 0.50 (
    +
0.1) in Ongole and Deoni breeds, respectively. The PIC values of the polymorphic loci ranged from 0.15 to 0.79 in Ongole and 0.13 to 0.80 in Deoni breeds. Six Ongole specific and three Deoni specific alleles were identified. The two breeds showed a moderate genetic relationship between themselves with a FST value of 0.10.

 Genetics