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1.
7132 Accesses
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Characterizing and measuring bias in sequence data
Michael G Ross, Carsten Russ, Maura Costello, Andrew Hollinger, Niall J Lennon, Ryan Hegarty, Chad Nusbaum, David B Jaffe Genome Biology 2013, 14:R51 (29 May 2013)
Abstract | Provisional PDF
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Editor’s summary
A comparison of the bias in sequencing data from four different platforms reveals that some human promoters are completelymissed
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2.
4742 Accesses
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The genome sequence of the most widely cultivated cacao type and its use to identify candidate genes regulating pod color
Juan C Motamayor, Keithanne Mockaitis, Jeremy Schmutz, Niina Haiminen, Donald Livingstone, Omar Cornejo, Seth D Findley, Ping Zheng, Filippo Utro, Stefan Royaert, Christopher Saski, Jerry Jenkins, Ram Podicheti, Meixia Zhao, Brian E Scheffler, Joseph C Stack, Frank A Feltus, Guiliana M Mustiga, Freddy Amores, Wilbert Phillips, Jean Philippe Marelli, Gregory D May, Howard Shapiro, Jianxin Ma, Carlos D Bustamante, Raymond J Schnell, Dorrie Main, Don Gilbert, Laxmi Parida, David N Kuhn et al.
Genome Biology 2013, 14:R53 (3 June 2013)
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Editor’s summary
The genome sequence of Theobroma cacao Matina 1-6 allows the identification of a gene associated with pod color
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3.
4475 Accesses
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Machine learning and genome annotation: a match meant to be?
Kevin Y Yip, Chao Cheng, Mark Gerstein Genome Biology 2013, 14:205 (29 May 2013)
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Mark Gerstein, Kevin Yip and Chao Cheng present a beginner's guide to machine learning for genome annotation
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4.
4078 Accesses
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REAPR: a universal tool for genome assembly evaluation
Martin Hunt, Taisei Kikuchi, Mandy Sanders, Chris Newbold, Matthew Berriman, Thomas D Otto Genome Biology 2013, 14:R47 (27 May 2013)
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Editor’s summary
A new user-friendly software tool for genome assembly evaluation
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5.
3949 Accesses
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Longitudinal, genome-scale analysis of DNA methylation in twins from birth to 18 months of age reveals rapid epigenetic change in early life and pair-specific effects of discordance
David Martino, Yuk Jin Loke, Lavinia Gordon, Miina Ollikainen, Mark N Cruickshank, Richard Saffery, Jeffrey M Craig Genome Biology 2013, 14:R42 (22 May 2013)
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Editor’s summary
A longitudinal study of DNA methylation in twins at birth and 18 months finds that some loci drift in methylation status
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6.
3857 Accesses
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TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
Daehwan Kim, Geo Pertea, Cole Trapnell, Harold Pimentel, Ryan Kelley, Steven L Salzberg Genome Biology 2013, 14:R36 (25 April 2013)
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Editor’s summary
A major update to TopHat, the popular Bowtie-based spliced aligner
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7.
3560 Accesses
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Genome of the long-living sacred lotus (Nelumbo nucifera Gaertn.)
Ray Ming, Robert VanBuren, Yanling Liu, Mei Yang, Yuepeng Han, Lei-Ting Li, Qiong Zhang, Min-Jeong Kim, Michael C Schatz, Michael Campbell, Jingping Li, John E Bowers, Haibao Tang, Eric Lyons, Ann A Ferguson, Giuseppe Narzisi, David R Nelson, Crysten E Blaby-Haas, Andrea R Gschwend, Yuannian Jiao, Joshua P Der, Fanchang Zeng, Jennifer Han, Xiang Jia Min, Karen A Hudson, Ratnesh Singh, Aleel K Grennan, Steven J Karpowicz, Jennifer R Watling, Kikukatsu Ito et al.
Genome Biology 2013, 14:R41 (10 May 2013)
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| F1000 Biology
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Editor’s summary
The genome of the long-living, slowly evolving sacred lotus reveals the genetic mechanisms underlying its adaptation to limited nutrient availability
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8.
2935 Accesses
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New gene expression pipelines gush lncRNAs
Jiashi Wang, Bronwyn A Lucas, Lynne E Maquat Genome Biology 2013, 14:117 (24 May 2013)
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Editor’s summary
The characterization of lncRNA expression during neuronal development is a useful resource for elucidating the functions of non-coding transcripts
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9.
2841 Accesses
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The plasticity of the grapevine berry transcriptome
Silvia Santo, Giovanni Tornielli, Sara Zenoni, Marianna Fasoli, Lorenzo Farina, Andrea Anesi, Flavia Guzzo, Massimo Delledonne, Mario Pezzotti Genome Biology 2013, 14:r54 (10 June 2013)
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Editor’s summary
A transcriptomic and metabolomic analysis of the plasticity of grapevine berry gene expression in response to development and the environment
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10.
2765 Accesses
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Differential expression analysis for sequence count data
Simon Anders, Wolfgang Huber Genome Biology 2010, 11:R106 (27 October 2010)
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| F1000 Biology
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Editor’s summary
DEseq allows the determination of differential expression of read count data from RNA-seq or ChIP-seq experiments
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11.
2721 Accesses
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Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
Ben Langmead, Cole Trapnell, Mihai Pop, Steven L Salzberg Genome Biology 2009, 10:R25 (4 March 2009)
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Editor’s summary
Bowtie: a new ultrafast memory-efficient tool for the alignment of short DNA sequence reads to large genomes.
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12.
2605 Accesses
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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman Genome Biology 2002, 3:research0034-research0034.11 (18 June 2002)
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Editor’s summary
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.
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13.
2495 Accesses
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Mutations within lncRNAs are effectively selected against in fruitfly but not in human
Wilfried Haerty, Chris P Ponting Genome Biology 2013, 14:R49 (27 May 2013)
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Editor’s summary
Analysis of lncRNA conservation in fruitfly and humans shows that these elements are well conserved in insects, but not in mammals
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14.
2494 Accesses
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DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus
Lucas JT Kaaij, Marc van de Wetering, Fang Fang, Benjamin Decato, Antoine Molaro, Harmen JG van de Werken, Johan H van Es, Jurian Schuijers, Elzo de Wit, Wouter de Laat, Gregory J Hannon, Hans C Clevers, Andrew D Smith, Rene F Ketting Genome Biology 2013, 14:R50 (28 May 2013)
Abstract | Provisional PDF
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Editor’s summary
Intriguingly, DNA methylation in mouse intestinal cells does not change much during differentiation
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15.
2492 Accesses
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Integrated analysis of recurrent properties of cancer genes to identify novel drivers
Matteo D'Antonio, Francesca D Ciccarelli Genome Biology 2013, 14:R52 (29 May 2013)
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Editor’s summary
A method to detect rare driver genes through integrated analysis of expression profiles, mutation effects and properties of known cancer genes
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16.
2404 Accesses
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Epigenetics and transgenerational inheritance
Emilie Brasset, Séverine Chambeyron Genome Biology 2013, 14:306 (24 May 2013)
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A report on the 'Non-coding RNA, epigenetics and transgenerational inheritance' meeting, held at Churchill College, Cambridge, UK, April 11-12, 2013.
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17.
2365 Accesses
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Characterizing the admixed African ancestry of African Americans
Fouad Zakharia, Analabha Basu, Devin Absher, Themistocles L Assimes, Alan S Go, Mark A Hlatky, Carlos Iribarren, Joshua W Knowles, Jun Li, Balasubramanian Narasimhan, Steven Sidney, Audrey Southwick, Richard M Myers, Thomas Quertermous, Neil Risch, Hua Tang Genome Biology 2009, 10:R141 (22 December 2009)
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| PubMed | Cited on BioMed Central
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Editor’s summary
Genome-wide SNP analyses reveal the admixed African genetic ancestry of African Americans.
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18.
2356 Accesses
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The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage
H Bradley Shaffer, Patrick Minx, Daniel E Warren, Andrew M Shedlock, Robert C Thomson, Nicole Valenzuela, John Abramyan, Chris T Amemiya, Daleen Badenhorst, Kyle K Biggar, Glen M Borchert, Christopher W Botka, Rachel M Bowden, Edward L Braun, Anne M Bronikowski, Benoit G Bruneau, Leslie T Buck, Blanche Capel, Todd A Castoe, Mike Czerwinski, Kim D Delehaunty, Scott V Edwards, Catrina C Fronick, Matthew K Fujita, Lucinda Fulton, Tina A Graves, Richard E Green, Wilfried Haerty, Ramkumar Hariharan, Omar Hernandez et al.
Genome Biology 2013, 14:R28 (28 March 2013)
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The genome of the western painted turtle, Chrysemys picta bellii, reveals insights into its extreme tolerance to anoxia and freezing
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19.
2230 Accesses
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Quartz-Seq: a highly reproducible and sensitive single-cell RNA-Seq reveals non-genetic gene expression heterogeneity
Yohei Sasagawa, Itoshi Nikaido, Tetsutaro Hayashi, Hiroki Danno, Kenichiro D Uno, Takeshi Imai, Hiroki R Ueda Genome Biology 2013, 14:R31 (17 April 2013)
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Quartz-Seq is a method for performing single cell RNA-seq which displays high reproducibility
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20.
2231 Accesses
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Insights into snoRNA biogenesis and processing from PAR-CLIP of snoRNA core proteins and small RNA sequencing
Shivendra Kishore, Andreas R Gruber, Dominik J Jedlinski, Afzal P Syed, Hadi Jorjani, Mihaela Zavolan Genome Biology 2013, 14:R45 (26 May 2013)
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PAR-CLIP of snoRNA-associated proteins reveals the existence of extensive human loci that are processed into snoRNAs
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21.
2184 Accesses
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After the gold rush
Neil Hall Genome Biology 2013, 14:115 (7 May 2013)
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Neil Hall wonders whether genomics is ready for a plateau in sequencing costs
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22.
2185 Accesses
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Adult monozygotic twins discordant for intra-uterine growth have indistinguishable genome-wide DNA methylation profiles
Nicole YP Souren, Pavlo Lutsik, Gilles Gasparoni, Sascha Tierling, Jasmin Gries, Matthias Riemenschneider, Jean-Pierre Fryns, Catherine Derom, Maurice P Zeegers, Jörn Walter Genome Biology 2013, 14:R44 (26 May 2013)
Abstract | Provisional PDF
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Editor’s summary
Monozygotic twins discordant for birth weight have very similar DNA methylation profiles
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23.
2141 Accesses
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Deep C diving: mapping the low-abundance modifications of the DNA demethylation pathway
John P Thomson, Jennifer M Hunter, Richard R Meehan Genome Biology 2013, 14:118 (29 May 2013)
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Editor’s summary
Richard Meehan and colleagues highlight recent insights into the 5th, 6th, 7th and 8th DNA bases: 5mC, 5hmC, 5fC and 5caC
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24.
1993 Accesses
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5-Hydroxymethylcytosine is an essential intermediate of active DNA demethylation processes in primary human monocytes
Maja Klug, Sandra Schmidhofer, Claudia Gebhard, Reinhard Andreesen, Michael Rehli Genome Biology 2013, 14:R46 (26 May 2013)
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TET2-mediated conversion of 5mC to 5hmC initiates active demethylation of the genome during monocyte differentiation
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25.
1790 Accesses
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Genome-wide analysis of functional sirtuin chromatin targets in yeast
Mingguang Li, Veena Valsakumar, Kunal Poorey, Stefan Bekiranov, Jeffrey S Smith Genome Biology 2013, 14:R48 (27 May 2013)
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Editor’s summary
Sirtuins regulate both glycolytic gene expression and condensin/cohesin deposition, suggesting a mechanistic link between metabolism and chromatin architecture
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26.
1647 Accesses
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CRISPR-Cas systems target a diverse collection of invasive mobile genetic elements in human microbiomes
Quan Zhang, Mina Rho, Haixu Tang, Thomas G Doak, Yuzhen Ye Genome Biology 2013, 14:R40 (29 April 2013)
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Editor’s summary
Analysis of CRISPR sequences contained in human metagenomic datasets reveals a variety of different targeted mobile genetic elements
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27.
1639 Accesses
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Genomic triumph meets clinical reality
Qasim Ayub, Yali Xue, Chris Tyler-Smith Genome Biology 2013, 14:307 (28 May 2013)
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Editor’s summary
A report on the 'Genomic Disorders 2013 - from 60 years of DNA to human genomes in the clinic' meeting, held at Homerton College, Hills Road, Cambridge, UK, April 10-12, 2013.
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28.
1561 Accesses
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Rising from the crypt: decreasing DNA methylation during differentiation of the small intestine
Sean M Cullen, Margaret A Goodell Genome Biology 2013, 14:116 (28 May 2013)
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Editor’s summary
A Research Highlight examines why differentiation in the small intestine crypt bypasses the DNA methylation changes observed in other lineage progressions
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29.
1449 Accesses
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Plastic, fantastic! Phenotypic variance in the transcriptional landscape of the grape berry
Alisdair R Fernie, Takayuki Tohge Genome Biology 2013, 14:119 (7 June 2013)
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Editor’s summary
A Research Highlight covering Mario Pezzotti's research article analyzing the plasticity of grape berrys in response to environmental changes
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30.
1441 Accesses
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A scaling normalization method for differential expression analysis of RNA-seq data
Mark D Robinson, Alicia Oshlack Genome Biology 2010, 11:R25 (2 March 2010)
Abstract | Full text | PDF
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Editor’s summary
A novel and empirical method for normalization of RNA-seq data is presented
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31.
1407 Accesses
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From RNA-seq reads to differential expression results
Alicia Oshlack, Mark D Robinson, Matthew D Young Genome Biology 2010, 11:220 (22 December 2010)
Abstract | Full text | PDF
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Editor’s summary
Many methods and tools are available for preprocessing high-throughput RNA sequencing data and detecting differential expression.
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32.
1385 Accesses
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Inactive or moderately active human promoters are enriched for inter-individual epialleles
Carolina Gemma, Sreeram V Ramagopalan, Thomas A Down, Huriya Beyan, Mohammed I Hawa, Michelle L Holland, Paul J Hurd, Gavin Giovannoni, R David Leslie, George C Ebers, Vardhman K Rakyan Genome Biology 2013, 14:R43 (25 May 2013)
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Editor’s summary
Epialleles between monozygotic twins are temporally stable, associated with chromatin changes and enriched in transcriptionally inactive regions
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33.
1357 Accesses
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The draft genome and transcriptome of Cannabis sativa
Harm van Bakel, Jake M Stout, Atina G Cote, Carling M Tallon, Andrew G Sharpe, Timothy R Hughes, Jonathan E Page Genome Biology 2011, 12:R102 (20 October 2011)
Abstract | Full text | PDF
| PubMed | Cited on BioMed Central |
| F1000 Biology
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Editor’s summary
The genome and transcriptome of marijuana, and comparative analysis with resequenced hemp, explains the genetic basis of psychoactivity
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34.
1254 Accesses
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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Daniel Aird, Michael G Ross, Wei-Sheng Chen, Maxwell Danielsson, Timothy Fennell, Carsten Russ, David B Jaffe, Chad Nusbaum, Andreas Gnirke Genome Biology 2011, 12:R18 (21 February 2011)
Abstract | Full text | PDF
| PubMed | Cited on BioMed Central
| F1000 Biology
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Editor’s summary
The optimization of PCR amplification steps in Illumina libraries significantly reduces sequencing bias
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35.
1232 Accesses
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Improving RNA-Seq expression estimates by correcting for fragment bias
Adam Roberts, Cole Trapnell, Julie Donaghey, John L Rinn, Lior Pachter Genome Biology 2011, 12:R22 (16 March 2011)
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Editor’s summary
An extension to Cufflinks corrects bias in RNA-seq datasets
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36.
1231 Accesses
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E Carpenter, Thouis R Jones, Michael R Lamprecht, Colin Clarke, In Kang, Ola Friman, David A Guertin, Joo Chang, Robert A Lindquist, Jason Moffat, Polina Golland, David M Sabatini Genome Biology 2006, 7:R100 (31 October 2006)
Abstract | Full text | PDF
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Editor’s summary
CellProfiler, the first free, open-source system for flexible and high-throughput cell image analysis is described.
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37.
1224 Accesses
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Raymond Gosling: the man who crystallized genes
Naomi Attar Genome Biology 2013, 14:402 (25 April 2013)
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"The most wonderful thing!" - Genome Biology meets Raymond Gosling, a key player in the discovery of the double helix
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38.
1219 Accesses
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Model-based Analysis of ChIP-Seq (MACS)
Yong Zhang, Tao Liu, Clifford A Meyer, Jérôme Eeckhoute, David S Johnson, Bradley E Bernstein, Chad Nusbaum, Richard M Myers, Myles Brown, Wei Li, X Shirley Liu Genome Biology 2008, 9:R137 (17 September 2008)
Abstract | Full text | PDF
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Editor’s summary
MACS performs model-based analysis of ChIP-Seq data generated by short read sequencers.
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39.
1175 Accesses
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Sixty years of genome biology
W Doolittle, Peter Fraser, Mark B Gerstein, Brenton R Graveley, Steven Henikoff, Curtis Huttenhower, Alicia Oshlack, Chris P Ponting, John L Rinn, Michael C Schatz, Jernej Ule, Detlef Weigel, George M Weinstock Genome Biology 2013, 14:113 (25 April 2013)
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Editor’s summary
Members of Genome Biology's Editorial Board select their highlights from the 60 years of genome biology that followed the double helix
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40.
1172 Accesses
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The Transcription Factor Encyclopedia
Dimas Yusuf, Stefanie L Butland, Magdalena I Swanson, Eugene Bolotin, Amy Ticoll, Warren A Cheung, Xiao Cindy Zhang, Christopher TD Dickman, Debra L Fulton, Jonathan S Lim, Jake M Schnabl, Oscar HP Ramos, Mireille Vasseur-Cognet, Charles N de Leeuw, Elizabeth M Simpson, Gerhart U Ryffel, Eric W-F Lam, Ralf Kist, Miranda SC Wilson, Raquel Marco-Ferreres, Jan J Brosens, Leonardo L Beccari, Paola Bovolenta, Bérénice A Benayoun, Lara J Monteiro, Helma DC Schwenen, Lars Grontved, Elizabeth Wederell, Susanne Mandrup, Reiner A Veitia et al.
Genome Biology 2012, 13:R24 (29 March 2012)
Abstract | Full text | PDF
| PubMed | Cited on BioMed Central |
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Editor’s summary
A high quality compendium of transcription factor data that allows researchers to add new data via a peer-review system
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41.
1142 Accesses
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How to evaluate a graduate studentship, or choosing the right doctoral advisor
Duncan T Odom Genome Biology 2013, 14:114 (29 April 2013)
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Editor’s summary
Duncan Odom has some sage advice for those considering PhD options
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42.
1127 Accesses
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A de novo assembly of the newt transcriptome combined with proteomic validation identifies new protein families expressed during tissue regeneration
Mario Looso, Jens Preussner, Konstantinos Sousounis, Marc Bruckskotten, Christian S Michel, Ettore Lignelli, Richard Reinhardt, Sabrina Hoeffner, Marcus Krueger, Panagiotis A Tsonis, Thilo Borchardt, Thomas Braun Genome Biology 2013, 14:R16 (20 February 2013)
Abstract | Provisional PDF
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Editor’s summary
A newt transcriptome, incorporating both undamaged and regenerating organs, reveals new protein families that may be involved in tissue regeneration
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43.
1120 Accesses
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Bioconductor: open software development for computational biology and bioinformatics
Robert C Gentleman, Vincent J Carey, Douglas M Bates, Ben Bolstad, Marcel Dettling, Sandrine Dudoit, Byron Ellis, Laurent Gautier, Yongchao Ge, Jeff Gentry, Kurt Hornik, Torsten Hothorn, Wolfgang Huber, Stefano Iacus, Rafael Irizarry, Friedrich Leisch, Cheng Li, Martin Maechler, Anthony J Rossini, Gunther Sawitzki, Colin Smith, Gordon Smyth, Luke Tierney, Jean YH Yang, Jianhua Zhang Genome Biology 2004, 5:R80 (15 September 2004)
Abstract | Full text | PDF
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Editor’s summary
A detailed description of the aims and methods of the Bioconductor project, an initiative for the collaborative creation of extensible software for computational biology and bioinformatics.
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44.
1096 Accesses
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Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment
Xochitl C Morgan, Timothy L Tickle, Harry Sokol, Dirk Gevers, Kathryn L Devaney, Doyle V Ward, Joshua A Reyes, Samir A Shah, Neal LeLeiko, Scott B Snapper, Athos Bousvaros, Joshua Korzenik, Bruce E Sands, Ramnik J Xavier, Curtis Huttenhower Genome Biology 2012, 13:R79 (26 September 2012)
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Editor’s summary
An analysis of the microbes and microbial metabolism present in intestinal biopsies and stool samples from 231 IBD and healthy subjects
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45.
1090 Accesses
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Functional analysis of transcription factor binding sites in human promoters
Troy W Whitfield, Jie Wang, Patrick J Collins, E Christopher Partridge, Shelley Aldred, Nathan D Trinklein, Richard M Myers, Zhiping Weng Genome Biology 2012, 13:R50 (5 September 2012)
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Editor’s summary
TFBSs are predicted from ENCODE data, and experiments in four cell lines find 70% to be functional
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46.
1082 Accesses
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Interactions between immunity, proliferation and molecular subtype in breast cancer prognosis
Srikanth Nagalla, Jeff W Chou, Mark C Willingham, Jimmy Ruiz, James P Vaughn, Purnima Dubey, Timothy L Lash, Stephen J Hamilton-Dutoit, Jonas Bergh, Christos Sotiriou, Michael A Black, Lance D Miller Genome Biology 2013, 14:R34 (29 April 2013)
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Editor’s summary
An analysis of nearly 2,000 breast cancer microarray samples reveals the presence of prognostic metagenes
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47.
1085 Accesses
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Comprehensive genotyping of the USA national maize inbred seed bank
Maria C Romay, Mark J Millard, Jeffrey C Glaubitz, Jason A Peiffer, Kelly L Swarts, Terry M Casstevens, Robert J Elshire, Charlotte B Acharya, Sharon E Mitchell, Sherry A Flint-Garcia, Michael D McMullen, James B Holland, Edward S Buckler, Candice A Gardner Genome Biology 2013, 14:R55 (11 June 2013)
Abstract | Provisional PDF
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Editor’s summary
Genotyping-by-Sequencing analysis of maize inbred accessions, identifying candidate genes for kernel color, sweet corn, and flowering time
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48.
1044 Accesses
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Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and Genome Analyzer systems
André E Minoche, Juliane C Dohm, Heinz Himmelbauer Genome Biology 2011, 12:R112 (8 November 2011)
Abstract | Full text | PDF
| PubMed | Cited on BioMed Central
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Editor’s summary
Error rates are compared in data from Illumina's HiSeq and GAIIx systems
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49.
1027 Accesses
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CRAC: an integrated approach to the analysis of RNA-seq reads
Nicolas Philippe, Mikaël Salson, Thérèse Commes, Eric Rivals Genome Biology 2013, 14:R30 (28 March 2013)
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Editor’s summary
Integrated analysis of mutations, indels and splice or chimeric junctions from RNA-seq data
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50.
1000 Accesses
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Conservation and divergence of transcriptomic and epigenomic variation in maize hybrids
Guangming He, Beibei Chen, Xuncheng Wang, Xueyong Li, Jigang Li, Hang He, Mei Yang, Lu Lu, Yijun Qi, Xiping Wang, Xing Wang Deng Genome Biology 2013, 14:R57 (12 June 2013)
Abstract | Provisional PDF
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Editor’s summary
An analysis of the effect of genotype and tissue type on transcriptional and epigenetic changes in maize inbred lines and hybrids
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