As a service to the research community, Genome Biology used to publish non-peer-reviewed articles in a 'preprint' depository which all individuals can access free of charge.From January 2006 Genome Biology no longer publishes new articles in this section. Authors of articles that were published in this depository have sole responsibility for their article's content. Each preprint has a permanent URL, by which it can be cited. Research submitted to the preprint depository may have been simultaneously or subsequently submitted to Genome Biology or any other publication for peer review; if possible, Genome Biology provides a reciprocal link from the preprint depository to the published article.

Deposited research article    

(28 December 2005)

It is shown that the topology of E. coli's metabolic network is sufficient to predict the viability of knock-out strains with accuracy comparable to flux balance analysis (FBA) on a large, unbiased dataset of mutants.

Deposited research article    

(19 December 2005)

Microarray analysis revealed that the expression levels of microRNA targets are significantly lower in all mouse and Drosophila tissues than in the embryos.

Deposited research article    

(11 October 2005)

Based on the bioinformatic identification of more than 200 additional members of known miRNA families in different species, an improved view of phylogenetic distribution, positional nucleotide preference, structural features and conservation of miRNA genes is presented. Furthermore, a classification of plant miRNA families is proposed and evidence for a common origin of the miR159 and miR319 families is presented.

Deposited research article    

(11 October 2005)

The frequency profiles of dinucleotides of the whole-genome sequence from 130 prokaryotic species (including archaea and bacteria) were analyzed. This study identified conserved frequencies of several dinucleotides (AC, AG, CA, CT, GA, GT, TC, and TG) and suggests that these frequencies may be evolutionary relics of the primordial genome.

Deposited research article    

(9 September 2005)

The e14 region from Escherichia coli is a decaying lambdoid prophage element. This study shows that a protein based comparative approach using BLAST can be used to identify similar lambdoid prophage elements in other bacterial genomes

Deposited research article    

(19 July 2005)

To trace admixture and genesis of caste populations of western India, polymorphisms were examined across non-recombining 20 Y-SNPs, 20 Y-STRs, 18 mtDNA diagnostic sites, HVS-1 plus HVS-2 regions; and recombining 15 highly polymorphic autosomal STRs in four predominant caste populations- upper-ranking Desasth-brahmin and Chitpavan-brahmin; a middle-ranking Kshtriya Maratha; and a lower-rank peasant Dhangar.