Your browser version may not work well with NCBI's Web applications. More information here...
1: Science. 2004 Nov 12;306(5699):1194-8.
Related Articles, Links
Click here to read
Multidimensional drug profiling by automated microscopy.

Perlman ZE, Slack MD, Feng Y, Mitchison TJ, Wu LF, Altschuler SJ.

Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiling at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs.

Publication Types:
PMID: 15539606 [PubMed - indexed for MEDLINE]