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1: RNA. 2005 Mar;11(3):355-8. Epub 2005 Jan 20.
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Small molecule control of pre-mRNA splicing.

Graveley BR.

Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA. graveley@neuron.uchc.edu

SR proteins regulate alternative splicing by binding to exonic sequences where, via an arginine/serine-rich splicing activation domain, they enhance the binding of the spliceosome to the adjacent splice sites. Here, a system is described in which a nontoxic derivative of the small molecule rapamycin is used to control pre-mRNA splicing in vitro. This involves the rapamycin-dependent recruitment of a splicing activation domain located on one protein to a second protein bound to the pre-mRNA. These results provide a new approach to explore for regulating gene expression in vivo with small molecules by controlling pre-mRNA splicing.

Publication Types:
PMID: 15661847 [PubMed - indexed for MEDLINE]

PMCID: PMC1370724