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1: Science. 2005 Aug 19;309(5738):1248-51. Epub 2005 Jul 14.
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Genome-wide RNAi screen for host factors required for intracellular bacterial infection.

Agaisse H, Burrack LS, Philips JA, Rubin EJ, Perrimon N, Higgins DE.

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA. herve.agaisse@yale.edu

Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.

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PMID: 16020693 [PubMed - indexed for MEDLINE]