Your browser version may not work well with NCBI's Web applications. More information here...
1: Science. 2006 May 19;312(5776):1044-6. Epub 2006 Apr 27.
Related Articles, Links
Click here to read Click here to read
Emergent properties of reduced-genome Escherichia coli.

Pósfai G, Plunkett G 3rd, Fehér T, Frisch D, Keil GM, Umenhoffer K, Kolisnychenko V, Stahl B, Sharma SS, de Arruda M, Burland V, Harcum SW, Blattner FR.

Institute of Biochemistry, Biological Research Center, H-6726 Szeged, Hungary. posfaigy@nucleus.szbk.u-szeged.hu

With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties.

Publication Types:
PMID: 16645050 [PubMed - indexed for MEDLINE]