Comment in:
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
Sawai S, Guan XJ, Kuspa A, Cox EC.
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. ssawai@complex.c.u-tokyo.ac.jp
We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.
Publication Types:
PMID: 17659086 [PubMed - indexed for MEDLINE]PMCID: PMC2323234