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A computer program for aligning a cDNA sequence with a genomic DNA sequence.

Florea L, Hartzell G, Zhang Z, Rubin GM, Miller W.

Department of Computer Science and Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802 USA.

We address the problem of efficiently aligning a transcribed and spliced DNA sequence with a genomic sequence containing that gene, allowing for introns in the genomic sequence and a relatively small number of sequencing errors. A freely available computer program, described herein, solves the problem for a 100-kb genomic sequence in a few seconds on a workstation.

Publication Types:
PMID: 9750195 [PubMed - indexed for MEDLINE]

PMCID: PMC310774