 | .reports |
For a comprehensive review of the most interesting recent articles published in the biological sciences, visit Faculty of 1000 Biology, an online literature awareness tool published by BioMed Central. Faculty of 1000 Biology systematically highlights exciting recent publications on the basis of recommendations of a faculty of well over 1,000 of the world's leading researchers. |
|
| Faculty Comments |
|
|
| Faculty Member |
Comments |
Michael Cappello
Yale University School of Medicine, United States of America
MICROBIOLOGY
 Confirmation
 New Finding
|
This article adds to our understanding of transcriptional regulation of gene expression in the trematode parasite Schistosoma mansoni. Using a previously developed biolistic transformation method, the authors demonstrate the role of genomic 5' flanking regions in directing tissue specific expression of GFP to the gut or tegument. This technique provides a method for comprehensive characterization of transcriptional regulation in parasitic helminths.
Evaluated 23 May 2005 |
|
|
|
| Faculty Comments |
|
|
| Faculty Member |
Comments |
Deyou Zheng
Yale University, United States of America
STRUCTURAL BIOLOGY
 Tech Advance
|
Here, the authors have constructed a new multi-species array in a great effort to address one of the commonly overlooked problems in applying microarray technology, namely the biasness of results when single-species arrays are used to directly compare interspecies gene expression. Microarray has been widely used for studying differential gene expression. Recently DNA arrays derived from the human genome or transcriptome have been applied to compare human gene expression patterns to those of chimp and other species. Results of such studies are very valuable; however, the authors in this study show that interspecies sequence divergence introduces significant artifacts and biases to conclusions obtained by those methods. These current findings indicate that naive use of single-species array to compare interspecies gene expression profiles can yield spurious results; instead, mutli-species microarrays should be the choice for such studies.
Evaluated 18 May 2005 |
|
|
|
| Faculty Comments |
|
|
| Faculty Member |
Comments |
Julia Kehr
Max-Planck-Institut fur Molekulare
PLANT BIOLOGY
New Finding
|
This is the first study describing a comprehensive experimental identification of micro RNAs (miRNAs) from a monocot plant species (rice) using a cloning approach. The experiments led to the finding of 21 known, but additionally to the discovery of 14 new rice miRNAs, of which, interestingly, only one was conserved in Arabidopsis. This suggests an independent evolution of mono- and dicot-specific miRNA-dependent gene regulatory processes.
Evaluated 17 May 2005 |
|
|
|
| Faculty Comments |
|
|
| Faculty Member |
Comments |
Roy Long
Medical College of Wisconsin, United States of America
CELL BIOLOGY
New Finding
|
Over twenty mRNAs are localized to the bud tip in S. cerevisiae, and this study is the first to identify a common structural motif within a subset of the cis-acting localization elements responsible for mRNA localization in this organism. Using a combination of genetics and bioinformatics, Olivier and colleagues determined that the RNA-binding protein She2p recognizes a loop-stem-loop structure which contains a conserved CGA triplet in one loop and a single conserved C residue in the other loop. The authors found this structure in the ASH1, IST2 and YMR171c cis-acting localization elements, and it remains to be determined if this structure is conserved in the other cis-acting localization elements sufficient for mRNA localization to the bud tip.
Evaluated 24 May 2005 |
|
|
 Recommended
|
|
F1000 Factor 3.0 |
|
|
|
Localization, annotation & comparison of the Escherichia coli K-12 proteome under two states of growth.
Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ
Mol Cell Proteomics 2005 May 18 [abstract on PubMed] [request from library] 
|
Selected by | Tracy Palmer
Evaluated 27 May 2005
|
|
|
|
| Faculty Comments |
|
|
| Faculty Member |
Comments |
Tracy Palmer
John Innes Centre, United Kingdom
MICROBIOLOGY
Confirmation
New Finding
|
This manuscript provides important new information on the compartmental localisation of E. coli proteins. The authors analyse the E. coli proteome by cell fractionation, 2D gel electrophoresis and mass spectrometry. They identify 575 different ORFs and show that many are post-translationally modified. This paper complements the work of others to characterise the E. coli proteome and provides a useful resource for many workers in the field.
Evaluated 27 May 2005 |
|
|
|
|
Must Read